Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI36310
Trapped Gene
Slc25a43 (
ENSMUSG00000037636
)
Vector Insertion
Chr X: 34284014 - 34292127
Public Clones
IST12044G5 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 27% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000355343 (ChrX:34283706..34284013 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GTCAGGATGGCTACGTGGAG
ChrX:34283733..34283752
60.68
60
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000355343 (ChrX:34283706..34284013 +)
Downstram Exon
ENSMUSE00000316538 (ChrX:34292128..34292369 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GTCAGGATGGCTACGTGGAG
ChrX:34283733..34283752
60.68
60
CGGGTTTTGATGAGGTCTGT
ChrX:34292253..34292272
59.97
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000355343
ChrX:34283706..34284013
GTCAGGATGGCTACGTGGAG
ChrX:34283733..34283752
60.68
60
*** Putative Vector Insertion (Chr X: 34284014 - 34292127) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000316538
ChrX:34292128..34292369
CGGGTTTTGATGAGGTCTGT
ChrX:34292253..34292272
59.97
50
downstream
ENSMUSE00000359857
ChrX:34297763..34297935
GGGGTCCATTCCAGACTTTT
ChrX:34297834..34297853
60.17
50
downstream
ENSMUSE00000394777
ChrX:34315477..34315611
CCAGTACCCCTTGGGTTTTC
ChrX:34315579..34315598
60.58
55
downstream
ENSMUSE00000370867
ChrX:34316997..34317197
GTGCAGTTTTCTGCGTTTGA
ChrX:34317179..34317198
60.03
45
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TGGTAGGGTAGGATGGGAAA
ChrX:34286965..34286985
59.24
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TGGTAGGGTAGGATGGGAAA
ChrX:34286965..34286985
59.24
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000037636