Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI36343
Trapped Gene
Maml1 (ENSMUSG00000050567)
Vector Insertion
Chr 11: 50079096 - 50105819
Public Clones IST11842F2 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000717356 (Chr11:50105228..50105818 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GTATAGCGGACAGGCCGAAG Chr11:50105764..50105783 62.02 60 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000717356 (Chr11:50105228..50105818 -)
Downstram Exon
ENSMUSE00000409149 (Chr11:50079097..50080512 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GTATAGCGGACAGGCCGAAG Chr11:50105764..50105783 62.02 60 CACGGGGTTCTGATTCAAGT Chr11:50079297..50079316 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000717356 Chr11:50105228..50105818 GTATAGCGGACAGGCCGAAG Chr11:50105764..50105783 62.02 60
upstream ENSMUSE00000718647 Chr11:50105228..50105818 GTATAGCGGACAGGCCGAAG Chr11:50105764..50105783 62.02 60
upstream ENSMUSE00000409149 Chr11:50079097..50080512 GCCCTCACGAGTGTGGTATT Chr11:50079683..50079702 60 55
upstream ENSMUSE00000679176 Chr11:50079097..50080512 GCCCTCACGAGTGTGGTATT Chr11:50079683..50079702 60 55

*** Putative Vector Insertion (Chr 11: 50079096 - 50105819) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000459430 Chr11:50076752..50076985 AGGGACACTGGAAGGGTTCT Chr11:50076940..50076959 59.97 55
downstream ENSMUSE00000296691 Chr11:50074479..50074575 GTTGTGGGAAGGTGCTTTGT Chr11:50074475..50074494 60.01 50
downstream ENSMUSE00000359511 Chr11:50069139..50072330 AGGCAGGGTTCCCTTACTGT Chr11:50071926..50071945 59.99 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTCCTGGGAACCAAGAATTT Chr11:50105817..50105838 59.8 38.1 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TACCCACGTGACTGGGAAAA Chr11:50105754..50105774 61.32 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000050567