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About
UniTrap UNI36525
Trapped Gene
Mast1 (
ENSMUSG00000053693
)
Vector Insertion
Chr 8: 87439353 - 87439702
Public Clones
IST13940F7 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 67% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000506540 (Chr8:87439579..87439701 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GCATCCTGAGGTGGTAGAGC
Chr8:87439590..87439609
59.83
60
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000506540 (Chr8:87439579..87439701 -)
Downstram Exon
ENSMUSE00000262112 (Chr8:87439354..87439490 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GCATCCTGAGGTGGTAGAGC
Chr8:87439590..87439609
59.83
60
CAATCCGGATGGAAGTGTTT
Chr8:87439411..87439430
59.79
45
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000581343
Chr8:87461094..87461252
GCTTTCCAATTTCTCGATGC
Chr8:87461134..87461153
59.79
45
upstream
ENSMUSE00000717225
Chr8:87461094..87461258
GCTTTCCAATTTCTCGATGC
Chr8:87461134..87461153
59.79
45
upstream
ENSMUSE00000485663
Chr8:87459605..87459693
GCACTTCTCCGACTCTACCG
Chr8:87459636..87459655
60.01
60
upstream
ENSMUSE00000581342
Chr8:87459143..87459218
CCCACTTCTCGTTTGCTTCT
Chr8:87459151..87459170
59.47
50
upstream
ENSMUSE00000262532
Chr8:87454719..87454797
No primer for this exon
upstream
ENSMUSE00000262507
Chr8:87453121..87453281
GGACGAGCTGCACTTTCTGT
Chr8:87453209..87453228
60.6
55
upstream
ENSMUSE00000581341
Chr8:87452849..87452924
CCTCCTCCTACGACAACGAA
Chr8:87452891..87452910
60.25
55
upstream
ENSMUSE00000517557
Chr8:87452479..87452688
GGCTCATCACCACGGTCTAT
Chr8:87452521..87452540
59.96
55
upstream
ENSMUSE00000516793
Chr8:87449544..87449645
CTCGGAGAGCTTGGAGGTAG
Chr8:87449615..87449634
59.18
60
upstream
ENSMUSE00000495053
Chr8:87449308..87449440
ACATCCCCCGATACATCATC
Chr8:87449342..87449361
59.44
50
upstream
ENSMUSE00000494005
Chr8:87449174..87449241
No primer for this exon
upstream
ENSMUSE00000496868
Chr8:87447927..87448012
ATTAGCAATGGTGCCTACGG
Chr8:87447927..87447946
59.98
50
upstream
ENSMUSE00000417078
Chr8:87447630..87447838
CGCCATGAAGAAGATCAACA
Chr8:87447777..87447796
59.8
45
upstream
ENSMUSE00000581340
Chr8:87445239..87445377
GAGATGGCGCGAATGTATTT
Chr8:87445311..87445330
60.07
45
upstream
ENSMUSE00000581339
Chr8:87444999..87445131
ATCAAGCTCACGGATTTTGG
Chr8:87445087..87445106
60.07
45
upstream
ENSMUSE00000581338
Chr8:87444696..87444861
GTGCCTTTCTTCGGGGATAC
Chr8:87444728..87444747
60.83
55
upstream
ENSMUSE00000713519
Chr8:87444530..87444615
TAGCCTCTTGCAGACCAACC
Chr8:87444535..87444554
60.4
55
upstream
ENSMUSE00000417055
Chr8:87444514..87444615
TAGCCTCTTGCAGACCAACC
Chr8:87444535..87444554
60.4
55
upstream
ENSMUSE00000581337
Chr8:87444259..87444381
TTCTTCCGAGACCTGGACTG
Chr8:87444333..87444352
60.38
55
upstream
ENSMUSE00000133882
Chr8:87442569..87442678
TCCTATGATGAGGACGACACC
Chr8:87442629..87442649
59.94
52.38
upstream
ENSMUSE00000417011
Chr8:87441952..87442130
CTCAGCACGAGCCTAAGACC
Chr8:87442086..87442105
60.16
60
upstream
ENSMUSE00000417006
Chr8:87441626..87441873
CCAAAGAGGCAGTTCAAGGA
Chr8:87441660..87441679
60.37
50
upstream
ENSMUSE00000468791
Chr8:87440452..87440658
TCAAGTCGGCTTCAGCTACA
Chr8:87440477..87440496
59.74
50
upstream
ENSMUSE00000507509
Chr8:87440055..87440284
GTGTCTACATGGGCGACTCC
Chr8:87440085..87440104
60.54
60
upstream
ENSMUSE00000506540
Chr8:87439579..87439701
GCATCCTGAGGTGGTAGAGC
Chr8:87439590..87439609
59.83
60
upstream
ENSMUSE00000262112
Chr8:87439354..87439490
CCACCACACCCTTTGAAAAC
Chr8:87439449..87439468
60.25
50
*** Putative Vector Insertion (Chr 8: 87439353 - 87439702) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000520050
Chr8:87437219..87437406
GACAAGGACCGGTTCAAAGA
Chr8:87437292..87437311
60.09
50
downstream
ENSMUSE00000635579
Chr8:87436989..87437140
CGGTACTGGCGGTGTAGTTT
Chr8:87436995..87437014
60.05
55
downstream
ENSMUSE00000635578
Chr8:87436945..87436986
GTGTGCGCTAGAGGTGACAG
Chr8:87436941..87436960
59.65
60
downstream
ENSMUSE00000635577
Chr8:87436899..87436943
No primer for this exon
downstream
ENSMUSE00000635576
Chr8:87436874..87436897
TAGTTTGGCAGGGAAGCTCT
Chr8:87436853..87436872
59.08
50
downstream
ENSMUSE00000681388
Chr8:87436858..87436872
No primer for this exon
downstream
ENSMUSE00000370543
Chr8:87435885..87436856
CACTGCGGGGTACATTCTTT
Chr8:87436009..87436028
59.99
50
downstream
ENSMUSE00000714349
Chr8:87435807..87435906
GCAGCATGGGCACTTTATTT
Chr8:87435786..87435805
60.1
45
downstream
ENSMUSE00000681389
Chr8:87435752..87437140
CACTGCGGGGTACATTCTTT
Chr8:87436009..87436028
59.99
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
GTAATCGCCTTGCAGCACAT
Chr8:87439632..87439652
61.2
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TTACAGCACGTGGAGGAAGG
Chr8:87439686..87439706
61.22
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000053693