Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI36572
Trapped Gene
AC121839.3-202 (ENSMUSG00000071224)
Vector Insertion
Chr 13: 99072583 - 99075265
Public Clones IST14844B4 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000611339 (Chr13:99072460..99072582 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACTTCTTGCGGGGTATTCCT Chr13:99072495..99072514 59.96 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000611339 (Chr13:99072460..99072582 +)
Downstram Exon
ENSMUSE00000611338 (Chr13:99075266..99075409 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACTTCTTGCGGGGTATTCCT Chr13:99072495..99072514 59.96 50 AACCCTGGTTGCTGTGAATC Chr13:99075314..99075333 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000680196 Chr13:99049893..99049933 GCAGATCGGCAGGAATAAAA Chr13:99049913..99049932 60.17 45
upstream ENSMUSE00000680194 Chr13:99053444..99053529 AGGGAGCATTGTTCACCAAA Chr13:99053457..99053476 60.49 45
upstream ENSMUSE00000680193 Chr13:99057213..99057276 CTTTGGATCGGCATCGTAAA Chr13:99057253..99057272 60.96 45
upstream ENSMUSE00000611339 Chr13:99072460..99072582 ACTTCTTGCGGGGTATTCCT Chr13:99072495..99072514 59.96 50

*** Putative Vector Insertion (Chr 13: 99072583 - 99075265) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000611338 Chr13:99075266..99075409 AACCCTGGTTGCTGTGAATC Chr13:99075314..99075333 59.97 50
downstream ENSMUSE00000611337 Chr13:99075676..99075798 CTGGTGTCACACACCCTGTC Chr13:99075769..99075788 60.04 60
downstream ENSMUSE00000611336 Chr13:99076911..99077047 ACTCCAGTCGGCACAGTCTT Chr13:99077029..99077048 59.91 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTCGGGACACCTACAAAACC Chr13:99072559..99072579 59.83 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTCGGGACACCTACAAAACC Chr13:99072559..99072579 59.83 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000071224