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About
UniTrap UNI36579
Trapped Gene
Arhgap26 (
ENSMUSG00000036452
)
Vector Insertion
Chr 18: 39387088 - 39389544
Public Clones
IST11889D12 (tigm)
IST11625F4 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000267794 (Chr18:39387020..39387087 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
GCCGCACAATTGGACAGTAT
Chr18:39387021..39387040
60.92
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000267794 (Chr18:39387020..39387087 +)
Downstram Exon
ENSMUSE00000659517 (Chr18:39389545..39389619 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
GCCGCACAATTGGACAGTAT
Chr18:39387021..39387040
60.92
50
ATCAGGACGCTCAGCAACTT
Chr18:39389620..39389639
60.02
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000659536
Chr18:39152799..39153283
GAGACGCTCAAGTCACACGA
Chr18:39153187..39153206
60.19
55
upstream
ENSMUSE00000714908
Chr18:39152799..39153283
GAGACGCTCAAGTCACACGA
Chr18:39153187..39153206
60.19
55
upstream
ENSMUSE00000659535
Chr18:39257284..39257379
GATGCCGAAACAGATGATGA
Chr18:39257349..39257368
59.61
45
upstream
ENSMUSE00000493448
Chr18:39259226..39259803
CTGCTAATGCCGAAGAAAGG
Chr18:39259673..39259692
59.97
50
upstream
ENSMUSE00000659534
Chr18:39259226..39259287
TCTTGAAGACGAGCGGAGTC
Chr18:39259263..39259282
60.68
55
upstream
ENSMUSE00000659533
Chr18:39261823..39261894
TTTCGAAAGGAGCAGATTGG
Chr18:39261865..39261884
60.32
45
upstream
ENSMUSE00000659532
Chr18:39270109..39270210
CTGCGGCACCTTAGAAAAAC
Chr18:39270147..39270166
59.88
50
upstream
ENSMUSE00000659531
Chr18:39271444..39271554
GGTTCGGCAACATTTCTACG
Chr18:39271464..39271483
60.5
50
upstream
ENSMUSE00000659530
Chr18:39279773..39279877
TTATGAGCTGGCCAAGGATT
Chr18:39279817..39279836
59.67
45
upstream
ENSMUSE00000659529
Chr18:39281428..39281557
AAGACCATCAGCCCGTACAC
Chr18:39281506..39281525
60
55
upstream
ENSMUSE00000659528
Chr18:39286487..39286587
ATGGTTCCATTCGACCAAAA
Chr18:39286552..39286571
60.17
40
upstream
ENSMUSE00000659527
Chr18:39291710..39291804
GACGAGTCCGTTACCCTCAA
Chr18:39291716..39291735
60.11
55
upstream
ENSMUSE00000659526
Chr18:39309694..39309772
GTTGTCGGAAGAGGACCGTA
Chr18:39309718..39309737
60.11
55
upstream
ENSMUSE00000659525
Chr18:39364938..39364974
CTCCAACAGAGACAGCCAGA
Chr18:39364946..39364965
59.12
55
upstream
ENSMUSE00000267794
Chr18:39387020..39387087
GCCGCACAATTGGACAGTAT
Chr18:39387021..39387040
60.92
50
upstream
ENSMUSE00000659524
Chr18:39387022..39387087
CCGCACAATTGGACAGTATC
Chr18:39387022..39387041
59
50
*** Putative Vector Insertion (Chr 18: 39387088 - 39389544) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000659517
Chr18:39389545..39389619
ATCAGGACGCTCAGCAACTT
Chr18:39389620..39389639
60.02
50
downstream
ENSMUSE00000659523
Chr18:39389545..39389619
ATCAGGACGCTCAGCAACTT
Chr18:39389620..39389639
60.02
50
downstream
ENSMUSE00000659516
Chr18:39403260..39403347
GAGCACTGGTGACGGTCTTT
Chr18:39403334..39403353
60.31
55
downstream
ENSMUSE00000659522
Chr18:39403260..39403347
GAGCACTGGTGACGGTCTTT
Chr18:39403334..39403353
60.31
55
downstream
ENSMUSE00000659515
Chr18:39404937..39404995
GGTACATCATGAGGGGTCCT
Chr18:39404968..39404987
58.67
55
downstream
ENSMUSE00000659521
Chr18:39404937..39404995
GGTACATCATGAGGGGTCCT
Chr18:39404968..39404987
58.67
55
downstream
ENSMUSE00000659514
Chr18:39406473..39406578
GAGCCGATGGACAAGACTGT
Chr18:39406531..39406550
60.27
55
downstream
ENSMUSE00000659520
Chr18:39406473..39406578
GAGCCGATGGACAAGACTGT
Chr18:39406531..39406550
60.27
55
downstream
ENSMUSE00000659513
Chr18:39446181..39446340
CAGGTTTGCCACAGTCATCA
Chr18:39446229..39446248
60.72
50
downstream
ENSMUSE00000659519
Chr18:39446181..39446340
CAGGTTTGCCACAGTCATCA
Chr18:39446229..39446248
60.72
50
downstream
ENSMUSE00000267685
Chr18:39456439..39456577
TTCTTCCGGGACAGGTGTAG
Chr18:39456503..39456522
60.1
55
downstream
ENSMUSE00000355575
Chr18:39466356..39466506
TGATGATGCTGTTCCTCTGC
Chr18:39466382..39466401
59.95
50
downstream
ENSMUSE00000465334
Chr18:39517207..39517482
AAAGTTGACGAGGAGGCTGA
Chr18:39517417..39517436
59.99
50
downstream
ENSMUSE00000467724
Chr18:39517207..39517317
GTGAAGTTGGGCTTGGATTC
Chr18:39517235..39517254
59.53
50
downstream
ENSMUSE00000395202
Chr18:39517453..39517482
AGTCTTCACGGACCGCTTCT
Chr18:39517481..39517500
61.36
55
downstream
ENSMUSE00000341650
Chr18:39522239..39522508
CTCGCAGATATGACGACCAA
Chr18:39522433..39522452
59.82
50
downstream
ENSMUSE00000423778
Chr18:39522771..39522862
TGTGAAGGAGAGCTCCGAGT
Chr18:39522843..39522862
60.13
55
downstream
ENSMUSE00000423781
Chr18:39522771..39522862
TGTGAAGGAGAGCTCCGAGT
Chr18:39522843..39522862
60.13
55
downstream
ENSMUSE00000468946
Chr18:39530728..39532165
GAGAGAAAGAGCGCCTGCTA
Chr18:39531288..39531307
60
55
downstream
ENSMUSE00000572654
Chr18:39530728..39532165
GAGAGAAAGAGCGCCTGCTA
Chr18:39531288..39531307
60
55
downstream
ENSMUSE00000659518
Chr18:39530728..39535939
GAGAGAAAGAGCGCCTGCTA
Chr18:39531288..39531307
60
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGGTCATAATCGCCTTGCAG
Chr18:39387133..39387153
60.24
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
GGCATTGTTCTCATGGAAGG
Chr18:39387116..39387136
60.46
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000036452