Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI36628
Trapped Gene
Tmem2 (ENSMUSG00000024754)
Vector Insertion
Chr 19: 21892502 - 21898277
Public Clones IST12513A9 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 55% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000252215 (Chr19:21892413..21892501 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CAAAGGTGTCAAAACGACCA Chr19:21892430..21892449 59.58 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000252215 (Chr19:21892413..21892501 +)
Downstram Exon
ENSMUSE00000252195 (Chr19:21898278..21898409 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CAAAGGTGTCAAAACGACCA Chr19:21892430..21892449 59.58 45 CAATGAGCCTGTCGATGATG Chr19:21898348..21898367 60.22 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000546673 Chr19:21852832..21853276 GCTGATTAGAGGGACCCTAGC Chr19:21852870..21852890 59.34 57.14
upstream ENSMUSE00000620765 Chr19:21854661..21854742 GAGAACTCTGAGCCGTCACC Chr19:21854682..21854701 59.99 60
upstream ENSMUSE00000642465 Chr19:21867128..21867470 ATCTGCTTCGCCATAACCAG Chr19:21867389..21867408 60.24 50
upstream ENSMUSE00000642464 Chr19:21871817..21871957 CAAAACCCTCGTCTCAGGAA Chr19:21871831..21871850 60.22 50
upstream ENSMUSE00000642463 Chr19:21872357..21872918 GGCAAGTCAGATGAACGTGA Chr19:21872500..21872519 59.84 50
upstream ENSMUSE00000642462 Chr19:21876352..21876521 TGGGCCTTAGTTGGTGTCAT Chr19:21876359..21876378 60.38 50
upstream ENSMUSE00000642461 Chr19:21881849..21882037 GCCGTTCTCAGGTCAAAGTC Chr19:21882014..21882033 59.85 55
upstream ENSMUSE00000252348 Chr19:21883759..21883928 GCACGTCGGTGAGATCATAG Chr19:21883775..21883794 59.27 55
upstream ENSMUSE00000252328 Chr19:21886257..21886466 CAGCTTTTCAAGGTGCATCA Chr19:21886421..21886440 59.99 45
upstream ENSMUSE00000353366 Chr19:21886701..21886906 TCATGCGTGATGGAGTGTTT Chr19:21886852..21886871 60.12 45
upstream ENSMUSE00000252251 Chr19:21887041..21887110 CCAACAACCACCTGATCAAT Chr19:21887070..21887089 58.26 45
upstream ENSMUSE00000252233 Chr19:21889921..21890049 CACTCCGCTGGGGATATTTT Chr19:21890001..21890020 61.19 50
upstream ENSMUSE00000252215 Chr19:21892413..21892501 CAAAGGTGTCAAAACGACCA Chr19:21892430..21892449 59.58 45

*** Putative Vector Insertion (Chr 19: 21892502 - 21898277) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000252195 Chr19:21898278..21898409 CAATGAGCCTGTCGATGATG Chr19:21898348..21898367 60.22 50
downstream ENSMUSE00000252178 Chr19:21899580..21899615 GTCAGCCCCTTTCCGTTATC Chr19:21899609..21899628 60.83 55
downstream ENSMUSE00000252158 Chr19:21900530..21900685 TCCTGACTGGACCCTTCATC Chr19:21900571..21900590 60.05 55
downstream ENSMUSE00000252137 Chr19:21904290..21904467 AGGCCCATCGTAAATCTGAA Chr19:21904332..21904351 59.53 45
downstream ENSMUSE00000252113 Chr19:21906517..21906732 GAGTTCTTATCGCCGTCCAG Chr19:21906593..21906612 59.84 55
downstream ENSMUSE00000252086 Chr19:21909908..21910116 CAACAGGCTGGTACTGTGGA Chr19:21910037..21910056 59.74 55
downstream ENSMUSE00000144752 Chr19:21916531..21916713 AACCTGAAAGCTCGTGTTGG Chr19:21916588..21916607 60.29 50
downstream ENSMUSE00000144754 Chr19:21919110..21919329 AATACTGCGGGTACGCTTTG Chr19:21919258..21919277 60.15 50
downstream ENSMUSE00000144757 Chr19:21922427..21922525 TCCTCGCTGAATTTCTGCTT Chr19:21922510..21922529 60.1 45
downstream ENSMUSE00000144755 Chr19:21926715..21926869 GAACATCCGCTTTTCCTTCA Chr19:21926816..21926835 60.19 45
downstream ENSMUSE00000144758 Chr19:21930149..21930252 GCCAGTCCCAGAACTGCTAA Chr19:21930229..21930248 60.4 55
downstream ENSMUSE00000546624 Chr19:21930645..21932817 CGCTCTGCGTTTTAAAGGTC Chr19:21931587..21931606 60.02 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TAATCGCCTTGCAGCACATC Chr19:21895553..21895573 62.25 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACCCAAGGGAGTACCTCTGC Chr19:21895466..21895486 60.51 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024754