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About
UniTrap UNI37140
Trapped Gene
Abca7 (
ENSMUSG00000035722
)
Vector Insertion
Chr 10: 79477933 - 79478050
Public Clones
CMHD-GT_112H6-3 (cmhd)
Private Clones
not available
Severity of mutation
(?)
Insertion after 98% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000312886 (Chr10:79477692..79477932 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000312886 (Chr10:79477692..79477932 +)
Downstram Exon
ENSMUSE00000365113 (Chr10:79478051..79478313 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
TCCTCTTCCCCTTGGTCTTT
Chr10:79478091..79478110
60.04
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000381185
Chr10:79460360..79460425
ACCTATCGACGGAGACAACC
Chr10:79460405..79460424
59.02
55
upstream
ENSMUSE00000313229
Chr10:79460514..79460607
ATCCTAGTGGCTGTCCGTCA
Chr10:79460559..79460578
60.68
55
upstream
ENSMUSE00000313224
Chr10:79460759..79460900
AGCCTGGGGTCCTGAGTAAC
Chr10:79460867..79460886
60.51
60
upstream
ENSMUSE00000313216
Chr10:79460989..79461101
No primer for this exon
upstream
ENSMUSE00000313204
Chr10:79461196..79461269
ACGACCACAGGAGAGTGACC
Chr10:79461197..79461216
60.16
60
upstream
ENSMUSE00000313199
Chr10:79461502..79461582
GGGTCAAGCCCAGGATTCTA
Chr10:79461522..79461541
61.35
55
upstream
ENSMUSE00000313189
Chr10:79462126..79462336
ATTGGTACGAAGCCAACCAG
Chr10:79462262..79462281
59.99
50
upstream
ENSMUSE00000313184
Chr10:79462426..79462565
ACTGGATGACCACCCTGTGT
Chr10:79462454..79462473
60.29
55
upstream
ENSMUSE00000313176
Chr10:79463457..79463573
GTGAACCAGACCTTCGAGGA
Chr10:79463457..79463476
60.24
55
upstream
ENSMUSE00000313168
Chr10:79463668..79463835
TGGCTGGAATCCTAGGACAA
Chr10:79463807..79463826
60.59
50
upstream
ENSMUSE00000313160
Chr10:79464235..79464464
GACCCATCCGAACTGTCATC
Chr10:79464361..79464380
60.33
55
upstream
ENSMUSE00000313152
Chr10:79464780..79464956
GTCCGTCAGCAGATCCTTTC
Chr10:79464794..79464813
59.81
55
upstream
ENSMUSE00000313145
Chr10:79465252..79465474
TGCGTTGCTGGTATTAGTGC
Chr10:79465450..79465469
59.9
50
upstream
ENSMUSE00000313139
Chr10:79465576..79465797
CGCCTATTTTGCGCTCTATC
Chr10:79465716..79465735
59.97
50
upstream
ENSMUSE00000313131
Chr10:79465877..79466078
GAAAGCCTGGCGCTACTAGA
Chr10:79465913..79465932
59.75
55
upstream
ENSMUSE00000313126
Chr10:79466542..79466652
GCCAGTATGGAATCCCTGAA
Chr10:79466542..79466561
59.89
50
upstream
ENSMUSE00000313120
Chr10:79467126..79467297
AAACATTTTCGTGGCTGTCC
Chr10:79467185..79467204
59.98
45
upstream
ENSMUSE00000313113
Chr10:79467464..79467595
GGCCATGATGTACAAACCAAC
Chr10:79467516..79467536
60.11
47.62
upstream
ENSMUSE00000313105
Chr10:79467699..79467838
GGAACGTCTGATACGGGATG
Chr10:79467774..79467793
60.34
55
upstream
ENSMUSE00000574927
Chr10:79468388..79468525
GTGTGGTCATCATGGACGAG
Chr10:79468439..79468458
59.96
55
upstream
ENSMUSE00000313086
Chr10:79468733..79468920
GCTGCGGTTACTACCTGACC
Chr10:79468859..79468878
59.76
60
upstream
ENSMUSE00000313077
Chr10:79468997..79469054
CCAGACGGGAAAAGAAGTCA
Chr10:79469013..79469032
60.22
50
upstream
ENSMUSE00000313071
Chr10:79469178..79469455
CACACGAGGAACCTCAGACA
Chr10:79469188..79469207
59.86
55
upstream
ENSMUSE00000313065
Chr10:79469631..79469667
TTCCTAAAGGTGGTGGAGGA
Chr10:79469634..79469653
59.52
50
upstream
ENSMUSE00000313057
Chr10:79469881..79469973
CCAGAGGCATCAGTTCTGGA
Chr10:79469940..79469959
61.36
55
upstream
ENSMUSE00000313046
Chr10:79470069..79470196
CTCCACAAGCGTTTTCTGCT
Chr10:79470143..79470162
60.57
50
upstream
ENSMUSE00000313034
Chr10:79470399..79470523
CCCTCAGGTCTCGTTCTTCA
Chr10:79470503..79470522
60.38
55
upstream
ENSMUSE00000313026
Chr10:79470608..79470706
No primer for this exon
upstream
ENSMUSE00000313018
Chr10:79470890..79471177
GGCCGAAATGTGTCTGACTT
Chr10:79471122..79471141
60.12
50
upstream
ENSMUSE00000313009
Chr10:79471284..79471316
AAGTGGGTGGATGAGGTCAG
Chr10:79471297..79471316
59.96
55
upstream
ENSMUSE00000313000
Chr10:79471408..79471585
ATGCGCTAGACCGTATCCTG
Chr10:79471515..79471534
60.26
55
upstream
ENSMUSE00000312992
Chr10:79471673..79471842
ACATGCCCTCCTACCATCTG
Chr10:79471741..79471760
59.95
55
upstream
ENSMUSE00000574926
Chr10:79472603..79472780
TTTGTCCCAGCCAGCTTTAC
Chr10:79472658..79472677
60.25
50
upstream
ENSMUSE00000312973
Chr10:79472896..79473011
GCCTTTCAGCAGAGAGCCTA
Chr10:79472941..79472960
59.86
55
upstream
ENSMUSE00000312965
Chr10:79473781..79473925
CACCTGCATCAACCTCTTCA
Chr10:79473853..79473872
59.83
50
upstream
ENSMUSE00000312957
Chr10:79474020..79474143
AGGGCTCATTGACATGGTTC
Chr10:79474088..79474107
59.93
50
upstream
ENSMUSE00000312947
Chr10:79474458..79474587
AGGGACCTCTGTTCCTCCTC
Chr10:79474527..79474546
59.66
60
upstream
ENSMUSE00000312937
Chr10:79474660..79474780
AGGGGGATGTGCTAGTCCTC
Chr10:79474746..79474765
60.48
60
upstream
ENSMUSE00000312929
Chr10:79474869..79474931
TTTACCGTGGGCAGAGGAAC
Chr10:79474870..79474889
62.32
55
upstream
ENSMUSE00000312921
Chr10:79476057..79476163
AGGGAAGACATCCACCTTCC
Chr10:79476086..79476105
60.31
55
upstream
ENSMUSE00000312913
Chr10:79476364..79476505
TCTGATGCCATCTTCGACCT
Chr10:79476416..79476435
60.77
50
upstream
ENSMUSE00000312906
Chr10:79476591..79476725
GTACCTACAGCGGAGGCAAC
Chr10:79476649..79476668
59.76
60
upstream
ENSMUSE00000312896
Chr10:79476974..79477077
GTAGTGCTCACGTCGCACAG
Chr10:79477058..79477077
60.68
60
upstream
ENSMUSE00000312892
Chr10:79477213..79477305
GTCTGGGAAGCTCTCAGCAT
Chr10:79477275..79477294
59.56
55
upstream
ENSMUSE00000312886
Chr10:79477692..79477932
No primer for this exon
*** Putative Vector Insertion (Chr 10: 79477933 - 79478050) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000365113
Chr10:79478051..79478313
TCCTCTTCCCCTTGGTCTTT
Chr10:79478091..79478110
60.04
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGCTGAGGTGCCTCTAATCG
Chr10:79477970..79477990
59.6
55
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TGAAGGGAAGAGCTGAGGTG
Chr10:79477960..79477980
60.52
55
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000035722