Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI3739
Trapped Gene
Kpna2 (ENSMUSG00000018362)
Vector Insertion
Chr 11: 106860672 - 106860684
Public Clones XS0540 (sanger) AD0675 (sanger) RST133 (baygenomics) YHA061 (baygenomics)
P106D04 (ggtc) P020C05 (ggtc) P102B11 (ggtc) M052B02 (ggtc) D062A02 (ggtc)
P119B11 (ggtc) W064D05 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000671093 (Chr11:106860673..106860683 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000671093 (Chr11:106860673..106860683 -)
Downstram Exon
ENSMUSE00000324280 (Chr11:106860673..106860778 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000324280 Chr11:106860673..106860778 No primer for this exon
upstream ENSMUSE00000671093 Chr11:106860673..106860683 No primer for this exon

*** Putative Vector Insertion (Chr 11: 106860672 - 106860684) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000324273 Chr11:106858552..106858649 No primer for this exon
downstream ENSMUSE00000716045 Chr11:106858552..106858649 No primer for this exon
downstream ENSMUSE00000337527 Chr11:106857823..106857960 No primer for this exon
downstream ENSMUSE00000671092 Chr11:106857529..106857960 No primer for this exon
downstream ENSMUSE00000382812 Chr11:106853917..106854005 No primer for this exon
downstream ENSMUSE00000401609 Chr11:106853172..106853440 No primer for this exon
downstream ENSMUSE00000108616 Chr11:106852727..106852821 No primer for this exon
downstream ENSMUSE00000108617 Chr11:106852383..106852646 No primer for this exon
downstream ENSMUSE00000367942 Chr11:106851953..106852186 No primer for this exon
downstream ENSMUSE00000481622 Chr11:106851503..106851685 No primer for this exon
downstream ENSMUSE00000482472 Chr11:106850638..106850787 No primer for this exon
downstream ENSMUSE00000324191 Chr11:106849950..106850293 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGCCGGCTGTACAGGTAATC Chr11:106860665..106860685 59.22 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTTTCGTGGAAATCTGAGC Chr11:106860682..106860702 59.81 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018362