Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37434
Trapped Gene
Mybbp1a (ENSMUSG00000040463)
Vector Insertion
Chr 11: 72263597 - 72264127
Public Clones PST21988-NR (escells) PST17847-NR (escells)
Private Clones not available
Severity of mutation (?) Insertion after 66% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000314559 (Chr11:72263495..72263596 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCACCAGAAGGAGCTATCCA Chr11:72263533..72263552 60.36 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000314559 (Chr11:72263495..72263596 +)
Downstram Exon
ENSMUSE00000314554 (Chr11:72264128..72264276 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCACCAGAAGGAGCTATCCA Chr11:72263533..72263552 60.36 55 CTTTGCTGCAGCTTCTGTTG Chr11:72264198..72264217 59.93 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000314731 Chr11:72254871..72255077 CTTCTGGGACATTGCGAAAC Chr11:72254993..72255012 60.64 50
upstream ENSMUSE00000314726 Chr11:72255180..72255275 CTGAAGCGCCTAATCACTGG Chr11:72255201..72255220 60.92 55
upstream ENSMUSE00000314717 Chr11:72255902..72255985 CAGCCTACAAGCCATGAACA Chr11:72255964..72255983 59.86 50
upstream ENSMUSE00000314708 Chr11:72256072..72256146 GCAAACCTTTTTGGAGTGCT Chr11:72256096..72256115 59.36 45
upstream ENSMUSE00000314699 Chr11:72256248..72256355 GCTCTGGTGGACATCCTCTC Chr11:72256332..72256351 59.8 60
upstream ENSMUSE00000314692 Chr11:72256978..72257153 TGAAAGTGATCCTCCGCTCT Chr11:72257030..72257049 59.95 50
upstream ENSMUSE00000314685 Chr11:72257243..72257410 AAGGAGCACAAGCTGCCTAA Chr11:72257283..72257302 60.15 50
upstream ENSMUSE00000314676 Chr11:72257505..72257622 AGACTTGATCCGCCATTTTG Chr11:72257580..72257599 60.07 45
upstream ENSMUSE00000314669 Chr11:72258184..72258479 AGAGCTATGTGGCCTGGTTG Chr11:72258371..72258390 60.28 55
upstream ENSMUSE00000651079 Chr11:72258184..72258332 TCTCCCTGTCATGCCTACCT Chr11:72258312..72258331 59.68 55
upstream ENSMUSE00000314661 Chr11:72258678..72258788 TGGATCATTTGCATCTGGAG Chr11:72258737..72258756 59.6 45
upstream ENSMUSE00000314654 Chr11:72259110..72259235 CCAGAGACGAAGCAGCACTT Chr11:72259162..72259181 60.73 55
upstream ENSMUSE00000314650 Chr11:72259396..72259563 CAGTTGGCAGACATGCTGTT Chr11:72259478..72259497 59.9 50
upstream ENSMUSE00000314643 Chr11:72259660..72259759 No primer for this exon
upstream ENSMUSE00000314637 Chr11:72260474..72260570 TTGCATCAAGAAAAGCATGG Chr11:72260515..72260534 59.81 40
upstream ENSMUSE00000392037 Chr11:72260651..72260802 CCCACCTTACTCCACGTTGT Chr11:72260765..72260784 59.88 55
upstream ENSMUSE00000314631 Chr11:72261055..72261141 TGGTCACTGATGACGCTGAT Chr11:72261098..72261117 60.28 50
upstream ENSMUSE00000314626 Chr11:72261232..72261384 AGTGAAGAGGAGGACCGTGA Chr11:72261301..72261320 59.83 55
upstream ENSMUSE00000314620 Chr11:72261458..72261628 GTGGAGTGGACAACGAGGAG Chr11:72261459..72261478 60.71 60
upstream ENSMUSE00000314612 Chr11:72261719..72261879 CACTGCTGAACGTGATCCAG Chr11:72261789..72261808 60.46 55
upstream ENSMUSE00000314603 Chr11:72262234..72262465 TGCCTCTGTCGCCCTATACT Chr11:72262342..72262361 59.86 55
upstream ENSMUSE00000314592 Chr11:72262545..72262676 CACTTACGGTCCCCATGTTC Chr11:72262633..72262652 60.23 55
upstream ENSMUSE00000676966 Chr11:72262658..72262676 No primer for this exon
upstream ENSMUSE00000314580 Chr11:72262763..72262831 No primer for this exon
upstream ENSMUSE00000314569 Chr11:72263222..72263335 CTGTCTGCACGAGAGCTGAG Chr11:72263252..72263271 60.07 60
upstream ENSMUSE00000314559 Chr11:72263495..72263596 GCACCAGAAGGAGCTATCCA Chr11:72263533..72263552 60.36 55

*** Putative Vector Insertion (Chr 11: 72263597 - 72264127) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000314554 Chr11:72264128..72264276 CTTTGCTGCAGCTTCTGTTG Chr11:72264198..72264217 59.93 50
downstream ENSMUSE00000386413 Chr11:72264379..72265050 TGTGACGCAGCATTCTTTTC Chr11:72264540..72264559 59.99 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGCAGTTCTAATCGCCTTGC Chr11:72263640..72263660 59.62 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector

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