Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37695
Trapped Gene
Tmem2 (ENSMUSG00000024754)
Vector Insertion
Chr 19: 21853277 - 21854660
Public Clones (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000546673 (Chr19:21852832..21853276 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
GCTGATTAGAGGGACCCTAGC Chr19:21852870..21852890 59.34 57.14 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000546673 (Chr19:21852832..21853276 +)
Downstram Exon
ENSMUSE00000620765 (Chr19:21854661..21854742 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
GCTGATTAGAGGGACCCTAGC Chr19:21852870..21852890 59.34 57.14 CTCATCCACAGGCTGACAGA Chr19:21854729..21854748 59.98 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000546673 Chr19:21852832..21853276 GCTGATTAGAGGGACCCTAGC Chr19:21852870..21852890 59.34 57.14

*** Putative Vector Insertion (Chr 19: 21853277 - 21854660) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000620765 Chr19:21854661..21854742 CTCATCCACAGGCTGACAGA Chr19:21854729..21854748 59.98 55
downstream ENSMUSE00000642465 Chr19:21867128..21867470 GGACGTAAGGGAACCACCTT Chr19:21867252..21867271 60.22 55
downstream ENSMUSE00000642464 Chr19:21871817..21871957 TTCCTGAGACGAGGGTTTTG Chr19:21871853..21871872 60.22 50
downstream ENSMUSE00000642463 Chr19:21872357..21872918 ATCCCCAAACACAAGCAGTC Chr19:21872379..21872398 59.97 50
downstream ENSMUSE00000642462 Chr19:21876352..21876521 CACCATCGATGACACCAACT Chr19:21876389..21876408 59.39 50
downstream ENSMUSE00000642461 Chr19:21881849..21882037 CACAATTCGGTCTCCAGCTT Chr19:21881949..21881968 60.26 50
downstream ENSMUSE00000252348 Chr19:21883759..21883928 AATGTTGCGGGTGAGAAGTC Chr19:21883844..21883863 60.12 50
downstream ENSMUSE00000252328 Chr19:21886257..21886466 GATGAACAGACAGGCCATCC Chr19:21886422..21886441 60.48 55
downstream ENSMUSE00000353366 Chr19:21886701..21886906 AGTGTGTCGAAGCCAATGGT Chr19:21886732..21886751 60.58 50
downstream ENSMUSE00000252251 Chr19:21887041..21887110 CAGCTGCGTTATTGATCAGG Chr19:21887102..21887121 59.44 50
downstream ENSMUSE00000252233 Chr19:21889921..21890049 ACTGGACTCCCCAGTTGGTT Chr19:21889974..21889993 60.8 55
downstream ENSMUSE00000252215 Chr19:21892413..21892501 TGGTCGTTTTGACACCTTTG Chr19:21892452..21892471 59.58 45
downstream ENSMUSE00000252195 Chr19:21898278..21898409 CAATGAGCCTGTCGATGATG Chr19:21898348..21898367 60.22 50
downstream ENSMUSE00000252178 Chr19:21899580..21899615 GTCAGCCCCTTTCCGTTATC Chr19:21899609..21899628 60.83 55
downstream ENSMUSE00000252158 Chr19:21900530..21900685 TCCTGACTGGACCCTTCATC Chr19:21900571..21900590 60.05 55
downstream ENSMUSE00000252137 Chr19:21904290..21904467 AGGCCCATCGTAAATCTGAA Chr19:21904332..21904351 59.53 45
downstream ENSMUSE00000252113 Chr19:21906517..21906732 GAGTTCTTATCGCCGTCCAG Chr19:21906593..21906612 59.84 55
downstream ENSMUSE00000252086 Chr19:21909908..21910116 CAACAGGCTGGTACTGTGGA Chr19:21910037..21910056 59.74 55
downstream ENSMUSE00000144752 Chr19:21916531..21916713 AACCTGAAAGCTCGTGTTGG Chr19:21916588..21916607 60.29 50
downstream ENSMUSE00000144754 Chr19:21919110..21919329 AATACTGCGGGTACGCTTTG Chr19:21919258..21919277 60.15 50
downstream ENSMUSE00000144757 Chr19:21922427..21922525 TCCTCGCTGAATTTCTGCTT Chr19:21922510..21922529 60.1 45
downstream ENSMUSE00000144755 Chr19:21926715..21926869 GAACATCCGCTTTTCCTTCA Chr19:21926816..21926835 60.19 45
downstream ENSMUSE00000144758 Chr19:21930149..21930252 GCCAGTCCCAGAACTGCTAA Chr19:21930229..21930248 60.4 55
downstream ENSMUSE00000546624 Chr19:21930645..21932817 CGCTCTGCGTTTTAAAGGTC Chr19:21931587..21931606 60.02 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGGGGAAGGTAAGGCTCCTC Chr19:21853270..21853290 60.94 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGGGGAAGGTAAGGCTCCTC Chr19:21853270..21853290 60.94 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000024754