Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37737
Trapped Gene
Pop1 (ENSMUSG00000022325)
Vector Insertion
Chr 15: 34437963 - 34438358
Public Clones (ggtc) (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 27% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000125602 (Chr15:34437875..34437962 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGAAAGCGCTCTCTCAAATG Chr15:34437927..34437946 59.3 45 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000125602 (Chr15:34437875..34437962 +)
Downstram Exon
ENSMUSE00000125610 (Chr15:34438359..34438546 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGAAAGCGCTCTCTCAAATG Chr15:34437927..34437946 59.3 45 CATCCTCTGGGGTCATCCTA Chr15:34438499..34438518 59.88 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000422489 Chr15:34425087..34425170 CTGTGGTGGGAGCTTCTAGG Chr15:34425099..34425118 59.86 60
upstream ENSMUSE00000254850 Chr15:34429044..34429187 GAAACCAGCCCAGTAACGTG Chr15:34429086..34429105 60.55 55
upstream ENSMUSE00000254839 Chr15:34432066..34432233 TTCTGGAACCTCAAGGCAGT Chr15:34432073..34432092 59.84 50
upstream ENSMUSE00000125612 Chr15:34434714..34434889 CTTCCTAGACGGTTGCAGGA Chr15:34434854..34434873 60.39 55
upstream ENSMUSE00000125614 Chr15:34436174..34436422 ACAAAGCCCGAAGATGTCAC Chr15:34436223..34436242 60.12 50
upstream ENSMUSE00000125602 Chr15:34437875..34437962 TGAAAGCGCTCTCTCAAATG Chr15:34437927..34437946 59.3 45

*** Putative Vector Insertion (Chr 15: 34437963 - 34438358) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000125610 Chr15:34438359..34438546 CATCCTCTGGGGTCATCCTA Chr15:34438499..34438518 59.88 55
downstream ENSMUSE00000125608 Chr15:34439518..34439774 CACTCTCCCCATCGTCTCTC Chr15:34439686..34439705 59.79 60
downstream ENSMUSE00000125609 Chr15:34440087..34440180 AGTGCGAAAGAGGGCCTATC Chr15:34440142..34440161 60.73 55
downstream ENSMUSE00000125611 Chr15:34441669..34441780 TCCTGCAAGTGTCAGTCCAC Chr15:34441726..34441745 59.87 55
downstream ENSMUSE00000125601 Chr15:34442487..34442606 TGGAATTTCTGCTGGTGATG Chr15:34442515..34442534 59.65 45
downstream ENSMUSE00000368966 Chr15:34442845..34442934 TTCCAATGAGCACAAGACCA Chr15:34442908..34442927 60.24 45
downstream ENSMUSE00000125607 Chr15:34445597..34445712 AGCTGCTTCACTCGCTCATT Chr15:34445621..34445640 60.31 50
downstream ENSMUSE00000125606 Chr15:34447582..34447773 TCACGAGCCCCTAAATCAAG Chr15:34447649..34447668 60.21 50
downstream ENSMUSE00000125605 Chr15:34455918..34456072 ACTCGTGCACCTCGGTAGAT Chr15:34455940..34455959 59.75 55
downstream ENSMUSE00000125604 Chr15:34458638..34458985 GCACCAGGCATTGAAGCTAT Chr15:34458781..34458800 60.24 50
downstream ENSMUSE00000683718 Chr15:34459618..34460402 TTCCACGCAGTTCCACAATA Chr15:34460376..34460395 60.11 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCGCTCTCTCAAATGTGTAGC Chr15:34437933..34437954 60.18 52.38 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATTTGCGTGACTGGGAAAAC Chr15:34438009..34438029 59.98 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000022325