Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37772
Trapped Gene
Rdm1 (ENSMUSG00000010362)
Vector Insertion
Chr 11: 101489379 - 101489586
Public Clones (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 11% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000113080 (Chr11:101489274..101489378 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000113080 (Chr11:101489274..101489378 +)
Downstram Exon
ENSMUSE00000113082 (Chr11:101489587..101489766 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000113080 Chr11:101489274..101489378 No primer for this exon

*** Putative Vector Insertion (Chr 11: 101489379 - 101489586) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000113082 Chr11:101489587..101489766 No primer for this exon
downstream ENSMUSE00000113079 Chr11:101491552..101491674 No primer for this exon
downstream ENSMUSE00000113085 Chr11:101492134..101492293 No primer for this exon
downstream ENSMUSE00000113083 Chr11:101495116..101495214 No primer for this exon
downstream ENSMUSE00000113081 Chr11:101496102..101496187 No primer for this exon
downstream ENSMUSE00000342882 Chr11:101497062..101497395 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TTTGTTGGTGTGGGATCTGA Chr11:101489331..101489351 59.94 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TTTTGTTGGTGTGGGATCTG Chr11:101489330..101489350 59.39 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000010362