Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37855
Trapped Gene
Mtap4 (ENSMUSG00000032479)
Vector Insertion
Chr 9: 109980589 - 109982284
Public Clones (ggtc) (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 91% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000583211 (Chr9:109980507..109980588 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AAGGTCTCCTCCAAGTGTGG Chr9:109980540..109980559 59.15 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000583211 (Chr9:109980507..109980588 +)
Downstram Exon
ENSMUSE00000583210 (Chr9:109982285..109982397 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AAGGTCTCCTCCAAGTGTGG Chr9:109980540..109980559 59.15 55 AAGTGGCCCACGTTATCAAG Chr9:109982378..109982397 59.99 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000690141 Chr9:109833962..109834444 CGCCTGACACTAGCATCCTT Chr9:109834002..109834021 60.42 55
upstream ENSMUSE00000583224 Chr9:109881354..109881595 GCAGTCGCAGTGGTACAGAA Chr9:109881354..109881373 60.06 55
upstream ENSMUSE00000583223 Chr9:109902276..109902344 TAGCCAATGGTGATCATGGA Chr9:109902303..109902322 59.88 45
upstream ENSMUSE00000583222 Chr9:109928605..109928727 AACTGGCCAGAAGATGCAAG Chr9:109928664..109928683 60.4 50
upstream ENSMUSE00000583221 Chr9:109930183..109930296 CACGAACGCTTCTGCATTTA Chr9:109930244..109930263 60.01 45
upstream ENSMUSE00000583220 Chr9:109934603..109934737 TCCACGGCTGTTGTATCTCA Chr9:109934629..109934648 60.26 50
upstream ENSMUSE00000583219 Chr9:109936877..109938010 ATGATACGACATCCCCCAAA Chr9:109937234..109937253 60.01 45
upstream ENSMUSE00000583218 Chr9:109939246..109939359 CCAGTCAAAGCTGCAGAACA Chr9:109939248..109939267 60.17 50
upstream ENSMUSE00000634040 Chr9:109954667..109956026 AGCGGGTATGTGATTTCAGG Chr9:109954901..109954920 59.96 50
upstream ENSMUSE00000690134 Chr9:109965521..109965627 GCAAAGGCTGCTGTAGGTGT Chr9:109965541..109965560 60.46 55
upstream ENSMUSE00000583216 Chr9:109966732..109966953 CAACATCGAAAGCCAAAACA Chr9:109966763..109966782 59.71 40
upstream ENSMUSE00000583215 Chr9:109969989..109970172 ACCGTTTCAAAAGCCACATC Chr9:109970082..109970101 59.98 45
upstream ENSMUSE00000583214 Chr9:109971028..109971087 CAAGACTGAGGGGAAACCTG Chr9:109971032..109971051 59.69 55
upstream ENSMUSE00000583213 Chr9:109971197..109971489 GAAGAGTGTTCGCTCCAAGG Chr9:109971426..109971445 59.99 55
upstream ENSMUSE00000219958 Chr9:109973530..109973643 CATTGCGAGTGCACAGAAAC Chr9:109973610..109973629 60.46 50
upstream ENSMUSE00000583212 Chr9:109975117..109975209 CCAAGTGTGGTTCCAAGGAC Chr9:109975160..109975179 60.4 55
upstream ENSMUSE00000583211 Chr9:109980507..109980588 AAGGTCTCCTCCAAGTGTGG Chr9:109980540..109980559 59.15 55

*** Putative Vector Insertion (Chr 9: 109980589 - 109982284) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000583210 Chr9:109982285..109982397 AAGTGGCCCACGTTATCAAG Chr9:109982378..109982397 59.99 50
downstream ENSMUSE00000529394 Chr9:109983925..109984114 ATCTGGCTGTCCAAGGTCTG Chr9:109984106..109984125 60.26 55
downstream ENSMUSE00000583202 Chr9:109984239..109986450 GGCTTCAGCCTGCAAACTAC Chr9:109985058..109985077 60.02 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCCTCTGTAGCAGGTGGACT Chr9:109980620..109980640 59.33 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTGTAGCAGGTGGACTCGTG Chr9:109980624..109980644 59.49 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000032479