Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI37965
Trapped Gene
Mov10 (ENSMUSG00000002227)
Vector Insertion
Chr 3: 104620853 - 104621075
Public Clones (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000671111 (Chr3:104620854..104621074 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000671111 (Chr3:104620854..104621074 -)
Downstram Exon
ENSMUSE00000714749 (Chr3:104620854..104621160 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000671114 Chr3:104621275..104621481 No primer for this exon
upstream ENSMUSE00000671112 Chr3:104621176..104621481 No primer for this exon
upstream ENSMUSE00000336505 Chr3:104620854..104621160 No primer for this exon
upstream ENSMUSE00000671111 Chr3:104620854..104621074 No primer for this exon
upstream ENSMUSE00000671113 Chr3:104620854..104621039 No primer for this exon
upstream ENSMUSE00000714749 Chr3:104620854..104621160 No primer for this exon

*** Putative Vector Insertion (Chr 3: 104620853 - 104621075) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000173475 Chr3:104607822..104608025 No primer for this exon
downstream ENSMUSE00000671146 Chr3:104607396..104607631 No primer for this exon
downstream ENSMUSE00000173474 Chr3:104607389..104607631 No primer for this exon
downstream ENSMUSE00000173473 Chr3:104607049..104607303 No primer for this exon
downstream ENSMUSE00000671143 Chr3:104607049..104607310 No primer for this exon
downstream ENSMUSE00000173482 Chr3:104605711..104605845 No primer for this exon
downstream ENSMUSE00000173486 Chr3:104605344..104605512 No primer for this exon
downstream ENSMUSE00000173485 Chr3:104604316..104604470 No primer for this exon
downstream ENSMUSE00000173480 Chr3:104603875..104604051 No primer for this exon
downstream ENSMUSE00000173487 Chr3:104603605..104603752 No primer for this exon
downstream ENSMUSE00000173490 Chr3:104603193..104603351 No primer for this exon
downstream ENSMUSE00000173481 Chr3:104602864..104602967 No primer for this exon
downstream ENSMUSE00000173483 Chr3:104602616..104602713 No primer for this exon
downstream ENSMUSE00000173489 Chr3:104602310..104602526 No primer for this exon
downstream ENSMUSE00000173488 Chr3:104600193..104600310 No primer for this exon
downstream ENSMUSE00000173477 Chr3:104599888..104600079 No primer for this exon
downstream ENSMUSE00000173478 Chr3:104599685..104599759 No primer for this exon
downstream ENSMUSE00000173479 Chr3:104598778..104598903 No primer for this exon
downstream ENSMUSE00000173472 Chr3:104598612..104598700 No primer for this exon
downstream ENSMUSE00000314720 Chr3:104598243..104598364 No primer for this exon
downstream ENSMUSE00000569816 Chr3:104597754..104598158 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000002227