Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI38037
Trapped Gene
Uty (ENSMUSG00000068457)
Vector Insertion
Chr Y: 439207 - 466455
Public Clones (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 100% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000626497 (ChrY:466313..466454 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TTGGCAGTTGAACGATATGAA ChrY:466420..466440 59.17 38.1 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000626497 (ChrY:466313..466454 -)
Downstram Exon
ENSMUSE00000626496 (ChrY:439208..439334 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TTGGCAGTTGAACGATATGAA ChrY:466420..466440 59.17 38.1 CCATGCCACAAAACCTCTTT ChrY:439229..439248 59.97 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000577901 ChrY:581850..582181 GAAGGCGCTTTGTGGATTAG ChrY:581854..581873 59.85 50
upstream ENSMUSE00000626519 ChrY:581599..581662 CAGCTTCTTCGGGTTCTTGA ChrY:581643..581662 60.51 50
upstream ENSMUSE00000626518 ChrY:576154..576262 GCTGAAGGAAAAGTGGAATCAG ChrY:576208..576229 60.24 45.46
upstream ENSMUSE00000626517 ChrY:560940..560989 No primer for this exon
upstream ENSMUSE00000626516 ChrY:535898..535956 No primer for this exon
upstream ENSMUSE00000626515 ChrY:533649..533769 TTTTTGCAGAGCCAAAGAAA ChrY:533708..533727 58.67 35
upstream ENSMUSE00000626514 ChrY:525779..525833 GGCCCTGATCGACTGTAATG ChrY:525803..525822 60.48 55
upstream ENSMUSE00000626513 ChrY:523217..523251 No primer for this exon
upstream ENSMUSE00000626512 ChrY:512934..513027 TTGCCATCACAAGTCAAAGC ChrY:512954..512973 59.85 45
upstream ENSMUSE00000626511 ChrY:511171..511297 No primer for this exon
upstream ENSMUSE00000441428 ChrY:510235..510271 No primer for this exon
upstream ENSMUSE00000626510 ChrY:507347..507445 GGAAGGTTCAGGATGCCTTT ChrY:507409..507428 60.44 50
upstream ENSMUSE00000626509 ChrY:506408..506627 CAGCTCGGAGCAAATCTTGT ChrY:506450..506469 60.54 50
upstream ENSMUSE00000626508 ChrY:505566..505700 CAATACCCGCAGAGCTTACC ChrY:505599..505618 59.73 55
upstream ENSMUSE00000626507 ChrY:504533..504628 TGTCCAGACAGCTTCACATCA ChrY:504585..504605 60.46 47.62
upstream ENSMUSE00000626506 ChrY:494350..495077 CCATTTCAAACTGGGTCCAC ChrY:495034..495053 60.21 50
upstream ENSMUSE00000626505 ChrY:490598..490727 ACCACCAACTTCGCCATATC ChrY:490645..490664 59.82 50
upstream ENSMUSE00000626504 ChrY:489212..489317 ACAGTAATTCGCGGTCTTGC ChrY:489229..489248 60.28 50
upstream ENSMUSE00000626503 ChrY:488391..488596 AACTGGGATCCCTCTGGAAC ChrY:488485..488504 60.31 55
upstream ENSMUSE00000626502 ChrY:474250..474314 No primer for this exon
upstream ENSMUSE00000626501 ChrY:474101..474175 TCTGGAAGGAGACAGAAAGCA ChrY:474154..474174 60.12 47.62
upstream ENSMUSE00000626500 ChrY:473291..473439 AGCTGACAAAGCTTCCTGCT ChrY:473400..473419 59.38 50
upstream ENSMUSE00000626499 ChrY:471315..471429 TGGGGTGTTCTGAATGACTTC ChrY:471323..471343 59.96 47.62
upstream ENSMUSE00000626498 ChrY:467479..467666 AGGCACTGTTCACTGGGTTC ChrY:467530..467549 60.16 55
upstream ENSMUSE00000626497 ChrY:466313..466454 TTGGCAGTTGAACGATATGAA ChrY:466420..466440 59.17 38.1
upstream ENSMUSE00000626496 ChrY:439208..439334 AAAGAGGTTTTGTGGCATGG ChrY:439251..439270 59.97 45

*** Putative Vector Insertion (Chr Y: 439207 - 466455) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000568719 ChrY:436025..436195 TTTCGTGCACAGTTCTGACA ChrY:436088..436107 59 45
downstream ENSMUSE00000704564 ChrY:433587..435016 TGCGTAAGTCTCCCAACACA ChrY:433592..433611 60.3 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTTAAAAGGCTGGGGCTGT ChrY:439436..439456 60.13 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTTAAAAGGCTGGGGCTGT ChrY:439436..439456 60.13 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000068457