Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI38412
Trapped Gene
Pcolce2 (ENSMUSG00000015354)
Vector Insertion
Chr 9: 95587260 - 95593279
Public Clones (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 69% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000219479 (Chr9:95587105..95587259 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000219479 (Chr9:95587105..95587259 +)
Downstram Exon
ENSMUSE00000219478 (Chr9:95593280..95593363 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000235944 Chr9:95538151..95538292 No primer for this exon
upstream ENSMUSE00000235915 Chr9:95539071..95539179 No primer for this exon
upstream ENSMUSE00000235892 Chr9:95570424..95570679 No primer for this exon
upstream ENSMUSE00000219483 Chr9:95578764..95578888 No primer for this exon
upstream ENSMUSE00000219484 Chr9:95581952..95582088 No primer for this exon
upstream ENSMUSE00000219479 Chr9:95587105..95587259 No primer for this exon

*** Putative Vector Insertion (Chr 9: 95587260 - 95593279) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000219478 Chr9:95593280..95593363 No primer for this exon
downstream ENSMUSE00000219481 Chr9:95595043..95595210 No primer for this exon
downstream ENSMUSE00000634936 Chr9:95595385..95595577 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 AGTAATCGCCTTGCAGCACA Chr9:95590279..95590299 61.9 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCGTCGAATTAGCGTGACTG Chr9:95590269..95590289 60.01 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000015354