Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI38563
Trapped Gene
Adat1 (ENSMUSG00000031949)
Vector Insertion
Chr 8: 114514104 - 114516149
Public Clones (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000579926 (Chr8:114516081..114516148 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CGCGTGGATAATAGGGTGTG Chr8:114516106..114516125 61.29 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000579926 (Chr8:114516081..114516148 -)
Downstram Exon
ENSMUSE00000710691 (Chr8:114514105..114514312 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CGCGTGGATAATAGGGTGTG Chr8:114516106..114516125 61.29 55 AAGGTCCACTCACGATTTGG Chr8:114514154..114514173 59.97 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000579926 Chr8:114516081..114516148 CGCGTGGATAATAGGGTGTG Chr8:114516106..114516125 61.29 55
upstream ENSMUSE00000579925 Chr8:114514105..114514312 CCAAATCGTGAGTGGACCTT Chr8:114514176..114514195 59.97 50
upstream ENSMUSE00000710691 Chr8:114514105..114514312 CCAAATCGTGAGTGGACCTT Chr8:114514176..114514195 59.97 50

*** Putative Vector Insertion (Chr 8: 114514104 - 114516149) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000214910 Chr8:114513751..114513819 TTGGACTGGCCTATGCATTT Chr8:114513743..114513762 60.47 45
downstream ENSMUSE00000214905 Chr8:114511723..114511777 CATGGCTGTCATTGAGGATG Chr8:114511732..114511751 60.07 50
downstream ENSMUSE00000214909 Chr8:114511022..114511152 CTGAGCCTCCACAGTCCTCT Chr8:114511041..114511060 59.58 60
downstream ENSMUSE00000214907 Chr8:114506062..114506665 GACATTCCACCTTGCCATCT Chr8:114506159..114506178 59.93 50
downstream ENSMUSE00000305933 Chr8:114503199..114503344 ACCCGACTGCTGTATTTCCA Chr8:114503256..114503275 60.52 50
downstream ENSMUSE00000305928 Chr8:114501939..114502038 TGTAACATCCAAGGGCTGCT Chr8:114501973..114501992 60.66 50
downstream ENSMUSE00000466491 Chr8:114496005..114496091 AGTCTGGCTGCTCGTCATCT Chr8:114495990..114496009 60.17 55
downstream ENSMUSE00000706274 Chr8:114495453..114496091 GCCAACCTGAGCAATGAGAT Chr8:114495725..114495744 60.23 50
downstream ENSMUSE00000719492 Chr8:114495453..114496062 GCCAACCTGAGCAATGAGAT Chr8:114495725..114495744 60.23 50
downstream ENSMUSE00000467515 Chr8:114492306..114492444 GTAGTCGGGTGGGTTTCTGA Chr8:114492299..114492318 59.97 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCGCGTGGATAATAGGGTGT Chr8:114516105..114516125 62.15 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000031949