Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI4049
Trapped Gene
Dus1l (ENSMUSG00000025155)
Vector Insertion
Chr 11: 120651047 - 120651156
Public Clones AQ0325 (sanger)
Private Clones not available
Severity of mutation (?) Insertion after 85% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000148372 (Chr11:120651157..120651194 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ATGCCAAGTGTGACCAGTGT Chr11:120651169..120651188 59.02 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000148372 (Chr11:120651157..120651194 -)
Downstram Exon
ENSMUSE00000148378 (Chr11:120650971..120651046 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ATGCCAAGTGTGACCAGTGT Chr11:120651169..120651188 59.02 50 TCTGAAAGCTCGCTTCTTGC Chr11:120650968..120650987 60.93 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000668736 Chr11:120657624..120657712 No primer for this exon
upstream ENSMUSE00000668739 Chr11:120657533..120657717 GCAGTCACAGACCTTCATCG Chr11:120657553..120657572 59.42 55
upstream ENSMUSE00000416093 Chr11:120657457..120657709 GCAGTCACAGACCTTCATCG Chr11:120657553..120657572 59.42 55
upstream ENSMUSE00000394010 Chr11:120656791..120657037 GAGCCCAGCTATGCTACACC Chr11:120656890..120656909 59.87 60
upstream ENSMUSE00000356468 Chr11:120656217..120656325 CCAATGACCCAGAGGTGTTT Chr11:120656299..120656318 59.82 50
upstream ENSMUSE00000309791 Chr11:120655582..120655632 GGAGGAGTGGGACCTTCTTC Chr11:120655591..120655610 60.05 60
upstream ENSMUSE00000148373 Chr11:120655148..120655260 CACGTGCAAAATTCGTGTCT Chr11:120655207..120655226 59.76 45
upstream ENSMUSE00000148376 Chr11:120654342..120654424 CTCCTGGGAACACATCAAGG Chr11:120654349..120654368 60.5 55
upstream ENSMUSE00000148375 Chr11:120654005..120654108 CTGTGGGAATCCCTGTGTTT Chr11:120654084..120654103 59.82 50
upstream ENSMUSE00000148377 Chr11:120653669..120653813 GGCAACACCCTTGTCCACTA Chr11:120653710..120653729 60.95 55
upstream ENSMUSE00000148380 Chr11:120653341..120653441 GCTAAAGCTTCGGTGTCAGG Chr11:120653344..120653363 60.01 55
upstream ENSMUSE00000148366 Chr11:120653169..120653274 TGAGGCCAGCTGACAACTTA Chr11:120653210..120653229 59.59 50
upstream ENSMUSE00000148374 Chr11:120652519..120652664 TGAGGAACCCTCACAAAACC Chr11:120652538..120652557 59.94 50
upstream ENSMUSE00000148372 Chr11:120651157..120651194 ATGCCAAGTGTGACCAGTGT Chr11:120651169..120651188 59.02 50

*** Putative Vector Insertion (Chr 11: 120651047 - 120651156) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000148378 Chr11:120650971..120651046 TCTGAAAGCTCGCTTCTTGC Chr11:120650968..120650987 60.93 50
downstream ENSMUSE00000668735 Chr11:120650520..120650873 TAGGGCACTACCCACGACTT Chr11:120650718..120650737 59.62 55
downstream ENSMUSE00000479139 Chr11:120650516..120650873 TAGGGCACTACCCACGACTT Chr11:120650718..120650737 59.62 55
downstream ENSMUSE00000668738 Chr11:120650515..120650873 TAGGGCACTACCCACGACTT Chr11:120650718..120650737 59.62 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGAAATCCAAAGGTGAGCTG Chr11:120651147..120651167 59.67 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGAAATCCAAAGGTGAGCTG Chr11:120651147..120651167 59.67 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000025155