Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI4851
Trapped Gene
Mat2b (ENSMUSG00000042032)
Vector Insertion
Chr 11: 40506148 - 40508304
Public Clones AH0143 (sanger) (sanger) XT0009 (sanger) RRS685 (baygenomics) E051A12 (ggtc)
E051A12 (ggtc) PST26097-NR (escells) IST15004C6 (tigm) IST14580H7 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000654336 (Chr11:40508305..40508325 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000654336 (Chr11:40508305..40508325 -)
Downstram Exon
ENSMUSE00000267725 (Chr11:40505980..40506147 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon GGGACGAAGTGGATGGAGAG Chr11:40505980..40505999 61.99 60

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000654336 Chr11:40508305..40508325 No primer for this exon

*** Putative Vector Insertion (Chr 11: 40506148 - 40508304) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000267725 Chr11:40505980..40506147 GGGACGAAGTGGATGGAGAG Chr11:40505980..40505999 61.99 60
downstream ENSMUSE00000679835 Chr11:40505980..40506130 GGGACGAAGTGGATGGAGAG Chr11:40505980..40505999 61.99 60
downstream ENSMUSE00000267719 Chr11:40501103..40501297 CCAGTGGCACCAGTAATGAG Chr11:40501226..40501245 59.16 55
downstream ENSMUSE00000679834 Chr11:40498988..40501297 CTAACACTTCACGGCAAGCA Chr11:40500768..40500787 60.05 50
downstream ENSMUSE00000267715 Chr11:40498729..40498843 AGCAGCATCTGGCTGACTCT Chr11:40498753..40498772 60.31 55
downstream ENSMUSE00000267710 Chr11:40498184..40498336 TCCAGGACTGCTTTTTCTCC Chr11:40498173..40498192 59.4 50
downstream ENSMUSE00000267702 Chr11:40495966..40496159 TGAACTGCACCTTGTCGAAC Chr11:40496045..40496064 59.88 50
downstream ENSMUSE00000267693 Chr11:40494158..40494271 ATTGCACACGCCATTTCATA Chr11:40494176..40494195 59.96 40
downstream ENSMUSE00000335852 Chr11:40492818..40493696 AATTTGGAGCAATCGAGCTG Chr11:40493610..40493629 60.35 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCGATGCGCTAAAATAATC Chr11:40508248..40508268 59.69 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGGCCATGGAACAGGTAAG Chr11:40508298..40508318 59.55 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 AGGCCATGGAACAGGTAAGA Chr11:40508297..40508317 59.55 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 AGGCCATGGAACAGGTAAGA Chr11:40508297..40508317 59.55 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000042032