Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI4903
Trapped Gene
Cct3 (ENSMUSG00000001416)
Vector Insertion
Chr 3: 88101189 - 88103263
Public Clones AS0982 (sanger) (sanger) CSI540 (baygenomics) CSI807 (baygenomics) D134G12 (ggtc)
3SD145H09 (ggtc) D009H10 (ggtc) 3SD040B10 (ggtc) D134G12 (ggtc)
P113C01 (ggtc) E114B05 (ggtc) 3SE040B11 (ggtc) D040B10 (ggtc) 5SD040B10 (ggtc)
IST15058F7 (tigm) IST15058F7 (tigm) IST11568B7 (tigm)
Private Clones OST274604 (lexicon) OST255993 (lexicon) OST249982 (lexicon) OST98915 (lexicon)
OST64156 (lexicon)
Severity of mutation (?) Insertion after 2% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000360495 (Chr3:88101061..88101188 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000360495 (Chr3:88101061..88101188 +)
Downstram Exon
ENSMUSE00000175674 (Chr3:88103264..88103325 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000360495 Chr3:88101061..88101188 No primer for this exon

*** Putative Vector Insertion (Chr 3: 88101189 - 88103263) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000175674 Chr3:88103264..88103325 No primer for this exon
downstream ENSMUSE00000175685 Chr3:88104722..88104772 No primer for this exon
downstream ENSMUSE00000175681 Chr3:88104851..88104913 No primer for this exon
downstream ENSMUSE00000175675 Chr3:88106690..88106786 No primer for this exon
downstream ENSMUSE00000175678 Chr3:88108935..88109052 No primer for this exon
downstream ENSMUSE00000175683 Chr3:88113083..88113269 No primer for this exon
downstream ENSMUSE00000175677 Chr3:88115595..88115744 No primer for this exon
downstream ENSMUSE00000175680 Chr3:88117164..88117296 No primer for this exon
downstream ENSMUSE00000292867 Chr3:88117478..88117559 No primer for this exon
downstream ENSMUSE00000175682 Chr3:88122268..88122448 No primer for this exon
downstream ENSMUSE00000175673 Chr3:88124382..88124627 No primer for this exon
downstream ENSMUSE00000175679 Chr3:88124936..88125067 No primer for this exon
downstream ENSMUSE00000175676 Chr3:88125366..88125688 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000001416