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About
UniTrap UNI5666
Trapped Gene
Ckap5 (
ENSMUSG00000040549
)
Vector Insertion
Chr 2: 91426074 - 91426394
Public Clones
CSI617 (baygenomics)
Private Clones
not available
Severity of mutation
(?)
Insertion after 52% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000449729 (Chr2:91425879..91426073 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CCTACACTCCGTTCCACTCC
Chr2:91425906..91425925
59.57
60
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000449729 (Chr2:91425879..91426073 +)
Downstram Exon
ENSMUSE00000449724 (Chr2:91426395..91426530 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CCTACACTCCGTTCCACTCC
Chr2:91425906..91425925
59.57
60
TTTGGCCTTTTCTAGCATGG
Chr2:91426442..91426461
60.2
45
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000643315
Chr2:91366994..91367007
No primer for this exon
upstream
ENSMUSE00000643314
Chr2:91386479..91386572
TGGGAGATGACAGTGAGTGG
Chr2:91386517..91386536
59.66
55
upstream
ENSMUSE00000399736
Chr2:91386490..91386572
TGGGAGATGACAGTGAGTGG
Chr2:91386517..91386536
59.66
55
upstream
ENSMUSE00000167374
Chr2:91388931..91389124
AATGCTCATGTTGCAGGAAA
Chr2:91389105..91389124
59.28
40
upstream
ENSMUSE00000390637
Chr2:91390709..91390915
CTGAAGGGCCTGGATAACAA
Chr2:91390845..91390864
60.07
50
upstream
ENSMUSE00000352788
Chr2:91395424..91395595
TGGAATCGAGATGCTGTGAA
Chr2:91395545..91395564
60.35
45
upstream
ENSMUSE00000167375
Chr2:91396657..91396789
CTAAGCCCAGTCGGTTTCTG
Chr2:91396703..91396722
59.87
55
upstream
ENSMUSE00000167380
Chr2:91397732..91397832
TGGATGCGGTAGAGATCCTT
Chr2:91397780..91397799
59.65
50
upstream
ENSMUSE00000371865
Chr2:91399206..91399319
GAAAAACCCCAAGCTGGAAG
Chr2:91399262..91399281
60.96
50
upstream
ENSMUSE00000519590
Chr2:91400964..91401068
TGTTGGAAAGGACACCAATG
Chr2:91400966..91400985
59.39
45
upstream
ENSMUSE00000687485
Chr2:91402911..91403000
No primer for this exon
upstream
ENSMUSE00000167373
Chr2:91403106..91403270
TTGCAAGAAGCTTTCGTCAC
Chr2:91403191..91403210
59.2
45
upstream
ENSMUSE00000167378
Chr2:91404477..91404605
CAGCTTTGAAGGTGGTTGGT
Chr2:91404532..91404551
60.15
50
upstream
ENSMUSE00000167392
Chr2:91405778..91405960
CCAAACCAGGACCGTTAAAA
Chr2:91405923..91405942
59.83
45
upstream
ENSMUSE00000167369
Chr2:91408294..91408407
GTGCCGGGACAAAGAATAAG
Chr2:91408346..91408365
59.57
50
upstream
ENSMUSE00000167382
Chr2:91408794..91408904
GCTGGCCTGTATGGAAGAAT
Chr2:91408877..91408896
59.15
50
upstream
ENSMUSE00000167390
Chr2:91410337..91410429
AATGGAGCGAACTGAGATGC
Chr2:91410348..91410367
60.37
50
upstream
ENSMUSE00000167371
Chr2:91412505..91412690
TGTGGGAACAATGCAAAAGA
Chr2:91412619..91412638
60.09
40
upstream
ENSMUSE00000449769
Chr2:91413130..91413224
GTCAATGGCCTTCTCACAAAA
Chr2:91413135..91413155
60.1
42.86
upstream
ENSMUSE00000449762
Chr2:91416173..91416230
CGTGAAGACTGCTCTTGCTG
Chr2:91416200..91416219
59.92
55
upstream
ENSMUSE00000449758
Chr2:91416320..91416445
CTGCTTGGCGTGATGTATCT
Chr2:91416344..91416363
58.9
50
upstream
ENSMUSE00000449749
Chr2:91417721..91417860
GATCTTTTGCCGAGGATTGA
Chr2:91417835..91417854
60.15
45
upstream
ENSMUSE00000449746
Chr2:91418192..91418366
TTGAAGGGTCGACTGAATGA
Chr2:91418331..91418350
59.22
45
upstream
ENSMUSE00000449741
Chr2:91419580..91419693
TGTAAAGAACCTGGGCATCC
Chr2:91419645..91419664
59.93
50
upstream
ENSMUSE00000449733
Chr2:91421267..91421395
AATGCTTGGGCAGAACAAAC
Chr2:91421300..91421319
60.12
45
upstream
ENSMUSE00000449729
Chr2:91425879..91426073
CCTACACTCCGTTCCACTCC
Chr2:91425906..91425925
59.57
60
*** Putative Vector Insertion (Chr 2: 91426074 - 91426394) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000449724
Chr2:91426395..91426530
TTTGGCCTTTTCTAGCATGG
Chr2:91426442..91426461
60.2
45
downstream
ENSMUSE00000449280
Chr2:91428826..91428914
AAGAAACGCCTGGAGCTTTA
Chr2:91428906..91428925
59.11
45
downstream
ENSMUSE00000449277
Chr2:91431823..91431948
TTTGCTTGGCACTTTCTTCC
Chr2:91431855..91431874
60.37
45
downstream
ENSMUSE00000385033
Chr2:91434840..91434989
TCATCCCGTGGGGTAGTAAA
Chr2:91434877..91434896
60.18
50
downstream
ENSMUSE00000301737
Chr2:91435468..91435671
AGGGGATAAAGGACGATGCT
Chr2:91435660..91435679
59.92
50
downstream
ENSMUSE00000301727
Chr2:91435845..91435980
CACATCCGGTTCAGGATAGC
Chr2:91435906..91435925
60.48
55
downstream
ENSMUSE00000301721
Chr2:91436519..91436712
TTCCGTACAGCATTGTCTCG
Chr2:91436645..91436664
59.86
50
downstream
ENSMUSE00000301714
Chr2:91439363..91439535
TGCTGACCGCTTTATCCTCT
Chr2:91439416..91439435
59.98
50
downstream
ENSMUSE00000301705
Chr2:91440798..91440971
TTTCACAGCGGACTGTACCA
Chr2:91440910..91440929
60.3
50
downstream
ENSMUSE00000301700
Chr2:91441163..91441283
TCAGAGCTTGGATGCTTGTG
Chr2:91441281..91441300
60.14
50
downstream
ENSMUSE00000301692
Chr2:91446403..91446582
TGATCAATGTGGCCAGACAT
Chr2:91446464..91446483
59.92
45
downstream
ENSMUSE00000391433
Chr2:91447602..91447789
TCACCACCAAGAGGTTCACA
Chr2:91447755..91447774
60.13
50
downstream
ENSMUSE00000301675
Chr2:91452897..91452972
AGCTGTTGCTAGCAGGCTGT
Chr2:91452942..91452961
60.36
55
downstream
ENSMUSE00000362729
Chr2:91455130..91455318
TGCTGTTGATGGTGTCAGGT
Chr2:91455178..91455197
60.16
50
downstream
ENSMUSE00000301668
Chr2:91455399..91455536
ATGCTTCATCATCCGACACA
Chr2:91455479..91455498
60.08
45
downstream
ENSMUSE00000301660
Chr2:91455951..91456034
TGGCCTTTGATGATTTTTCA
Chr2:91455975..91455994
59.09
35
downstream
ENSMUSE00000380899
Chr2:91456458..91456617
GCCTTTGCTTTCTCTCTCCA
Chr2:91456601..91456620
59.69
50
downstream
ENSMUSE00000687475
Chr2:91459845..91459996
GCACACATGTGACCTCCATC
Chr2:91459880..91459899
59.97
55
downstream
ENSMUSE00000336116
Chr2:91459908..91459996
GTAGACAGATGGCCCCACTT
Chr2:91459948..91459967
59.02
55
downstream
ENSMUSE00000373938
Chr2:91460139..91460819
CAACTGGAGGAATTGCTGGT
Chr2:91460199..91460218
60.11
50
downstream
ENSMUSE00000687474
Chr2:91460139..91460820
CAACTGGAGGAATTGCTGGT
Chr2:91460199..91460218
60.11
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
ATAATCGCCTTGCAGCACAT
Chr2:91426124..91426144
60.64
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
ATGGCTAAGGCTACTGGGAAA
Chr2:91426048..91426069
60.1
47.62
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000040549