Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI5671
Trapped Gene
Myst2 (ENSMUSG00000038909)
Vector Insertion
Chr 11: 95155960 - 95161292
Public Clones XC316 (baygenomics) CSI661 (baygenomics) XJ101 (baygenomics) F052A06 (ggtc)
PST2433-NR (escells) PST11963-NR (escells) PST11963-NL (escells) PST2885-NR (escells)
PST12581-NL (escells) PST4534-NR (escells) PST1217 (escells) PST12482-NR (escells)
PST15020-NR (escells) PST1309 (escells)
Private Clones not available
Severity of mutation (?) Insertion after 32% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000648581 (Chr11:95161293..95161532 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGATGAGTCACCACCTCGAA Chr11:95161491..95161510 60.25 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000648581 (Chr11:95161293..95161532 -)
Downstram Exon
ENSMUSE00000648580 (Chr11:95155877..95155959 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGATGAGTCACCACCTCGAA Chr11:95161491..95161510 60.25 50 TATGATACAGCGGGCATCCT Chr11:95155878..95155897 60.46 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000661432 Chr11:95171349..95171515 AGGGTTTGGGACAGCTACAG Chr11:95171450..95171469 59.21 55
upstream ENSMUSE00000674260 Chr11:95170851..95170916 CGGATAGAGATGGCGATAGG Chr11:95170861..95170880 59.65 55
upstream ENSMUSE00000674263 Chr11:95170851..95170871 No primer for this exon
upstream ENSMUSE00000445467 Chr11:95167363..95167510 GGAACCGAAGATTCCGATTT Chr11:95167467..95167486 60.26 45
upstream ENSMUSE00000577649 Chr11:95164447..95164623 GAAGGGTGACCCGTAGTCAG Chr11:95164544..95164563 59.57 60
upstream ENSMUSE00000648581 Chr11:95161293..95161532 TGATGAGTCACCACCTCGAA Chr11:95161491..95161510 60.25 50

*** Putative Vector Insertion (Chr 11: 95155960 - 95161292) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000648580 Chr11:95155877..95155959 TATGATACAGCGGGCATCCT Chr11:95155878..95155897 60.46 50
downstream ENSMUSE00000577648 Chr11:95152834..95152923 GTTGCATGCCTGTTGTTGTC Chr11:95152822..95152841 60.17 50
downstream ENSMUSE00000648578 Chr11:95151086..95151184 ACCGCAGCTGTCTCTCTGTT Chr11:95151138..95151157 60.21 55
downstream ENSMUSE00000648577 Chr11:95147644..95147754 AGAGAGGCTCCCGAGTGTTC Chr11:95147693..95147712 60.93 60
downstream ENSMUSE00000648576 Chr11:95145343..95145534 GATTTGGCCTTGAAGCCTTA Chr11:95145486..95145505 59.3 45
downstream ENSMUSE00000648574 Chr11:95143153..95143242 GATGGAGCCTTTGCGATAAA Chr11:95143164..95143183 60.17 45
downstream ENSMUSE00000648573 Chr11:95142815..95142955 CAGCCAGTATTGTCCGCTTC Chr11:95142818..95142837 60.8 55
downstream ENSMUSE00000648572 Chr11:95141705..95141798 GATCAGCATCTTCCCGTAGC Chr11:95141690..95141709 59.8 55
downstream ENSMUSE00000648571 Chr11:95138783..95138929 TGGAGAGCCGACTTTCTCTT Chr11:95138867..95138886 59.16 50
downstream ENSMUSE00000648570 Chr11:95137757..95137863 ACGGGATTCACAGCTGTTTC Chr11:95137811..95137830 60.12 50
downstream ENSMUSE00000648569 Chr11:95135574..95137201 CTTGCCAATCACAAGAGCAA Chr11:95136984..95137003 59.99 45
downstream ENSMUSE00000413313 Chr11:95135573..95137201 CTTGCCAATCACAAGAGCAA Chr11:95136984..95137003 59.99 45

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTGTTGTGTAATCGCCTTG Chr11:95161230..95161250 59.19 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CATACACCGTGACTGGGAAA Chr11:95161228..95161248 59.42 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector
PCR 3 TAGAGGGACCCACTCACCAG Chr11:95161555..95161575 60.1 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 4 TAGAGGGACCCACTCACCAG Chr11:95161555..95161575 60.1 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000038909