Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI6239
Trapped Gene
Sall1 (
ENSMUSG00000031665
)
Vector Insertion
Chr 8: 91557298 - 91566250
Public Clones
(sanger)
(sanger)
CSG464 (baygenomics)
E067B05 (ggtc)
D142D01 (ggtc)
E140G05 (ggtc)
E067B05 (ggtc)
D124C03 (ggtc)
E140G05 (ggtc)
D152B09 (ggtc)
IST10122B11BBR1 (tigm)
IST15052E12 (tigm)
IST13010D6 (tigm)
IST10122D11 (tigm)
IST11229G2 (tigm)
IST12587C5 (tigm)
IST10122A11BBR1 (tigm)
IST12318F12 (tigm)
IST12506C5 (tigm)
IST13321E2 (tigm)
IST10122D12 (tigm)
IST10122C11 (tigm)
IST10654H10 (tigm)
IST14793B10 (tigm)
IST14810B2 (tigm)
IST12587C5 (tigm)
Private Clones
not available
Severity of mutation
(?)
Insertion after 2% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000243454 (Chr8:91566251..91566343 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
CAAGCGAAGCCTCAACATTT
Chr8:91566292..91566311
60.39
45
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000243454 (Chr8:91566251..91566343 -)
Downstram Exon
ENSMUSE00000211596 (Chr8:91553843..91557297 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
CAAGCGAAGCCTCAACATTT
Chr8:91566292..91566311
60.39
45
GGGGTGGGAGATAGACCATT
Chr8:91554464..91554483
60.01
55
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000488541
Chr8:91567929..91568061
AACTAAGCCGAGGACCAAGC
Chr8:91567998..91568017
60.76
55
upstream
ENSMUSE00000243454
Chr8:91566251..91566343
CAAGCGAAGCCTCAACATTT
Chr8:91566292..91566311
60.39
45
*** Putative Vector Insertion (Chr 8: 91557298 - 91566250) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000211596
Chr8:91553843..91557297
GGGGTGGGAGATAGACCATT
Chr8:91554464..91554483
60.01
55
downstream
ENSMUSE00000243441
Chr8:91551147..91552717
CAGAGATCTCGTTGGCCTTC
Chr8:91552470..91552489
59.95
55
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AGTAATCGCCTTGCAGCACA
Chr8:91557278..91557298
61.9
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TGAAAACTGGGATTGTGGTG
Chr8:91566237..91566257
59.39
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
PCR 3
AACCCTGCACCTTTTGATGT
Chr8:91557302..91557322
59.45
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 4
AACCCTGCACCTTTTGATGT
Chr8:91557302..91557322
59.45
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000031665