Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI627
Trapped Gene
Zfp273 (ENSMUSG00000030446)
Vector Insertion
Chr 13: 67914834 - 67923177
Public Clones AL0615 (sanger) AY0282 (sanger) AK0183 (sanger) IST10669G9 (tigm)
IST12295H11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000613394 (Chr13:67914770..67914833 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TCAAAGTTCGGACAGTGTGG Chr13:67914789..67914808 59.72 50 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000613394 (Chr13:67914770..67914833 +)
Downstram Exon
ENSMUSE00000570390 (Chr13:67923178..67923304 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TCAAAGTTCGGACAGTGTGG Chr13:67914789..67914808 59.72 50 TCCACTGAGCAGAGTCCAGA Chr13:67923256..67923275 59.69 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000613394 Chr13:67914770..67914833 TCAAAGTTCGGACAGTGTGG Chr13:67914789..67914808 59.72 50

*** Putative Vector Insertion (Chr 13: 67914834 - 67923177) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000570390 Chr13:67923178..67923304 TCCACTGAGCAGAGTCCAGA Chr13:67923256..67923275 59.69 55
downstream ENSMUSE00000494938 Chr13:67924037..67924132 CCAGGTATGGCTTGGAGAAA Chr13:67924066..67924085 60.07 50
downstream ENSMUSE00000517030 Chr13:67925921..67927937 TCCTTCCCGAGTCATTATGC Chr13:67927520..67927539 60.04 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CTGAGGTTTAATCGCCTTGC Chr13:67914877..67914897 59.85 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGGTTCGTGACTGGGAAAA Chr13:67914879..67914899 60.09 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000030446