Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI628
Trapped Gene
Prep (ENSMUSG00000019849)
Vector Insertion
Chr 10: 44840647 - 44845769
Public Clones AY0248 (sanger) IST10835A5 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 57% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000098545 (Chr10:44840449..44840646 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000098545 (Chr10:44840449..44840646 +)
Downstram Exon
ENSMUSE00000098546 (Chr10:44845770..44845873 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000354490 Chr10:44787012..44787181 No primer for this exon
upstream ENSMUSE00000098559 Chr10:44791859..44791933 No primer for this exon
upstream ENSMUSE00000098548 Chr10:44810799..44810932 No primer for this exon
upstream ENSMUSE00000098556 Chr10:44812468..44812598 No primer for this exon
upstream ENSMUSE00000098558 Chr10:44814787..44814996 No primer for this exon
upstream ENSMUSE00000098553 Chr10:44817199..44817320 No primer for this exon
upstream ENSMUSE00000098552 Chr10:44827578..44827683 No primer for this exon
upstream ENSMUSE00000386296 Chr10:44834869..44835060 No primer for this exon
upstream ENSMUSE00000098545 Chr10:44840449..44840646 No primer for this exon

*** Putative Vector Insertion (Chr 10: 44840647 - 44845769) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000098546 Chr10:44845770..44845873 No primer for this exon
downstream ENSMUSE00000098551 Chr10:44867791..44867927 No primer for this exon
downstream ENSMUSE00000098547 Chr10:44870242..44870336 No primer for this exon
downstream ENSMUSE00000440456 Chr10:44872861..44872992 No primer for this exon
downstream ENSMUSE00000440452 Chr10:44875322..44875478 No primer for this exon
downstream ENSMUSE00000643791 Chr10:44878038..44878803 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ATAATCGCCTTGCAGCACAT Chr10:44843697..44843717 60.64 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGTGTGCAGTGGGCTAACCT Chr10:44843638..44843658 59.79 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000019849