Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI6798
Trapped Gene
Sp2 (
ENSMUSG00000018678
)
Vector Insertion
Chr 11: 96815885 - 96817088
Public Clones
RHA041 (baygenomics)
(ggtc)
Private Clones
OST44823 (lexicon)
OST40030 (lexicon)
Severity of mutation
(?)
Insertion after 95% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000111019 (Chr11:96817089..96817282 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000111019 (Chr11:96817089..96817282 -)
Downstram Exon
ENSMUSE00000585965 (Chr11:96815533..96815884 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000673954
Chr11:96844167..96844273
No primer for this exon
upstream
ENSMUSE00000673953
Chr11:96842726..96842899
No primer for this exon
upstream
ENSMUSE00000673962
Chr11:96838935..96839025
No primer for this exon
upstream
ENSMUSE00000673971
Chr11:96838935..96839002
No primer for this exon
upstream
ENSMUSE00000673959
Chr11:96829902..96829979
No primer for this exon
upstream
ENSMUSE00000371569
Chr11:96824679..96824758
No primer for this exon
upstream
ENSMUSE00000673958
Chr11:96824679..96824755
No primer for this exon
upstream
ENSMUSE00000673970
Chr11:96824679..96824758
No primer for this exon
upstream
ENSMUSE00000414411
Chr11:96822352..96823323
No primer for this exon
upstream
ENSMUSE00000111024
Chr11:96818738..96819050
No primer for this exon
upstream
ENSMUSE00000111023
Chr11:96817419..96817593
No primer for this exon
upstream
ENSMUSE00000111019
Chr11:96817089..96817282
No primer for this exon
*** Putative Vector Insertion (Chr 11: 96815885 - 96817088) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000585965
Chr11:96815533..96815884
No primer for this exon
downstream
ENSMUSE00000673957
Chr11:96814787..96815884
No primer for this exon
downstream
ENSMUSE00000673960
Chr11:96814655..96815884
No primer for this exon
downstream
ENSMUSE00000673964
Chr11:96814654..96815884
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CATGCCCTTGTCTGTCCTTT
Chr11:96817056..96817076
60.11
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CATGCCCTTGTCTGTCCTTT
Chr11:96817056..96817076
60.11
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
PCR 3
CCAACTCCTCCCCCTACTCT
Chr11:96817297..96817317
59.55
60
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 4
CCAACTCCTCCCCCTACTCT
Chr11:96817297..96817317
59.55
60
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000018678
