Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI7106
Trapped Gene
Tjap1 (
ENSMUSG00000012296
)
Vector Insertion
Chr 17: 46401928 - 46419294
Public Clones
RRJ368 (baygenomics)
YTC803 (baygenomics)
D015E06 (ggtc)
D106H07 (ggtc)
D137A08 (ggtc)
D015E06 (ggtc)
D106H07 (ggtc)
PST7408-NR (escells)
PST6814-NR (escells)
IST14251E2 (tigm)
IST10442F7 (tigm)
IST14640A12 (tigm)
IST14915C9 (tigm)
IST14808A7 (tigm)
Private Clones
OST347652 (lexicon)
OST257851 (lexicon)
OST176619 (lexicon)
OST43734 (lexicon)
Severity of mutation
(?)
Insertion after 0% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000222842 (Chr17:46419295..46419388 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000222842 (Chr17:46419295..46419388 -)
Downstram Exon
ENSMUSE00000136622 (Chr17:46401832..46401927 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000398694
Chr17:46419909..46419948
No primer for this exon
upstream
ENSMUSE00000222842
Chr17:46419295..46419388
No primer for this exon
*** Putative Vector Insertion (Chr 17: 46401928 - 46419294) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000136622
Chr17:46401832..46401927
No primer for this exon
downstream
ENSMUSE00000136626
Chr17:46400638..46400762
No primer for this exon
downstream
ENSMUSE00000136629
Chr17:46399351..46399379
No primer for this exon
downstream
ENSMUSE00000136624
Chr17:46398368..46398529
No primer for this exon
downstream
ENSMUSE00000136628
Chr17:46398085..46398151
No primer for this exon
downstream
ENSMUSE00000136625
Chr17:46397044..46397151
No primer for this exon
downstream
ENSMUSE00000136627
Chr17:46396886..46396969
No primer for this exon
downstream
ENSMUSE00000136623
Chr17:46394826..46396432
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
AAGATGCAGTGCCACCTTTT
Chr17:46416283..46416303
59.74
45
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
AAGATGCAGTGCCACCTTTT
Chr17:46416283..46416303
59.74
45
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
PCR 3
CCAGGGACTGGAAGAAATCA
Chr17:46407349..46407369
60.04
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 4
CCAGGGACTGGAAGAAATCA
Chr17:46407349..46407369
60.04
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000012296
