Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI7141
Trapped Gene
Tgfbr1 (ENSMUSG00000007613)
Vector Insertion
Chr 4: 47419925 - 47423523
Public Clones RRU402 (baygenomics) RRJ579 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 90% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000178300 (Chr4:47419794..47419924 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000178300 (Chr4:47419794..47419924 +)
Downstram Exon
ENSMUSE00000527314 (Chr4:47423524..47425005 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000178301 Chr4:47366177..47366268 No primer for this exon
upstream ENSMUSE00000674233 Chr4:47366179..47366268 No primer for this exon
upstream ENSMUSE00000178302 Chr4:47396621..47396866 No primer for this exon
upstream ENSMUSE00000632705 Chr4:47399280..47399361 No primer for this exon
upstream ENSMUSE00000230734 Chr4:47406119..47406361 No primer for this exon
upstream ENSMUSE00000674231 Chr4:47406131..47406361 No primer for this exon
upstream ENSMUSE00000527316 Chr4:47409227..47409457 No primer for this exon
upstream ENSMUSE00000178298 Chr4:47415675..47415842 No primer for this exon
upstream ENSMUSE00000178299 Chr4:47416242..47416398 No primer for this exon
upstream ENSMUSE00000527315 Chr4:47418401..47418525 No primer for this exon
upstream ENSMUSE00000178300 Chr4:47419794..47419924 No primer for this exon

*** Putative Vector Insertion (Chr 4: 47419925 - 47423523) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000475457 Chr4:47423524..47427795 No primer for this exon
downstream ENSMUSE00000527314 Chr4:47423524..47425005 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TATAATCGCCTTGCAGCACA Chr4:47422974..47422994 60.38 45 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 AGATGGCAGAGCTGTGAGGT Chr4:47419908..47419928 60.02 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000007613