Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI7245
Trapped Gene
Taf6l (ENSMUSG00000003680)
Vector Insertion
Chr 19: 8850009 - 8852576
Public Clones RRT133 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 50% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000144057 (Chr19:8852577..8852709 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000144057 (Chr19:8852577..8852709 -)
Downstram Exon
ENSMUSE00000144059 (Chr19:8849880..8850008 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000234142 Chr19:8860700..8860753 No primer for this exon
upstream ENSMUSE00000234119 Chr19:8859612..8859668 No primer for this exon
upstream ENSMUSE00000234094 Chr19:8858325..8858484 No primer for this exon
upstream ENSMUSE00000144064 Chr19:8857794..8857880 No primer for this exon
upstream ENSMUSE00000144065 Chr19:8857034..8857184 No primer for this exon
upstream ENSMUSE00000144066 Chr19:8856846..8856896 No primer for this exon
upstream ENSMUSE00000144062 Chr19:8856491..8856585 No primer for this exon
upstream ENSMUSE00000144060 Chr19:8853341..8853415 No primer for this exon
upstream ENSMUSE00000144061 Chr19:8853003..8853223 No primer for this exon
upstream ENSMUSE00000144057 Chr19:8852577..8852709 No primer for this exon

*** Putative Vector Insertion (Chr 19: 8850009 - 8852576) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000144059 Chr19:8849880..8850008 No primer for this exon
downstream ENSMUSE00000233870 Chr19:8848984..8849780 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTGGAAGGTGAGGACTCTG Chr19:8852563..8852583 59.99 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GCTGGAAGGTGAGGACTCTG Chr19:8852563..8852583 59.99 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000003680