Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI7595
Trapped Gene
Map3k4 (ENSMUSG00000014426)
Vector Insertion
Chr 17: 12457015 - 12463728
Public Clones RRR430 (baygenomics) RRN121 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 36% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000556030 (Chr17:12463729..12465086 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000556030 (Chr17:12463729..12465086 -)
Downstram Exon
ENSMUSE00000135609 (Chr17:12456772..12457014 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000503769 Chr17:12511364..12511614 No primer for this exon
upstream ENSMUSE00000248177 Chr17:12470787..12470977 No primer for this exon
upstream ENSMUSE00000556030 Chr17:12463729..12465086 No primer for this exon

*** Putative Vector Insertion (Chr 17: 12457015 - 12463728) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000135609 Chr17:12456772..12457014 No primer for this exon
downstream ENSMUSE00000248099 Chr17:12454110..12454256 No primer for this exon
downstream ENSMUSE00000248070 Chr17:12453317..12453474 No primer for this exon
downstream ENSMUSE00000248052 Chr17:12450780..12450896 No primer for this exon
downstream ENSMUSE00000135594 Chr17:12449628..12449727 No primer for this exon
downstream ENSMUSE00000135589 Chr17:12449457..12449540 No primer for this exon
downstream ENSMUSE00000135600 Chr17:12447863..12448129 No primer for this exon
downstream ENSMUSE00000135598 Chr17:12446948..12447097 No primer for this exon
downstream ENSMUSE00000135603 Chr17:12442363..12442524 No primer for this exon
downstream ENSMUSE00000135588 Chr17:12441770..12441903 No primer for this exon
downstream ENSMUSE00000135593 Chr17:12440919..12440997 No primer for this exon
downstream ENSMUSE00000135597 Chr17:12440169..12440235 No primer for this exon
downstream ENSMUSE00000135604 Chr17:12436366..12436462 No primer for this exon
downstream ENSMUSE00000135592 Chr17:12435516..12435671 No primer for this exon
downstream ENSMUSE00000135591 Chr17:12432836..12432936 No primer for this exon
downstream ENSMUSE00000247757 Chr17:12432245..12432325 No primer for this exon
downstream ENSMUSE00000247740 Chr17:12430905..12431096 No primer for this exon
downstream ENSMUSE00000247722 Chr17:12429847..12429917 No primer for this exon
downstream ENSMUSE00000247699 Chr17:12428765..12428881 No primer for this exon
downstream ENSMUSE00000135601 Chr17:12427917..12428076 No primer for this exon
downstream ENSMUSE00000135587 Chr17:12425780..12425902 No primer for this exon
downstream ENSMUSE00000135596 Chr17:12425278..12425384 No primer for this exon
downstream ENSMUSE00000247627 Chr17:12422383..12422562 No primer for this exon
downstream ENSMUSE00000703857 Chr17:12420489..12420986 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCAGAAAGTAATCGCCTTGC Chr17:12460665..12460685 59.99 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGTATTTAAGCCCGCGTGA Chr17:12460672..12460692 60.48 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000014426