Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI7660
Trapped Gene
Dnm2 (ENSMUSG00000033335)
Vector Insertion
Chr 9: 21299062 - 21303701
Public Clones RRS014 (baygenomics) (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 64% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000638331 (Chr9:21298948..21299061 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TTCGTGCTGACAGCTGAGTC Chr9:21299017..21299036 60.34 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000638331 (Chr9:21298948..21299061 +)
Downstram Exon
ENSMUSE00000638330 (Chr9:21303702..21303811 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TTCGTGCTGACAGCTGAGTC Chr9:21299017..21299036 60.34 55 AAACACGTGCTTGTTGGACA Chr9:21303791..21303810 60.2 45

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000476895 Chr9:21229415..21229700 ACATCGGAACCGAGAGTCTT Chr9:21229487..21229506 58.74 50
upstream ENSMUSE00000584899 Chr9:21261947..21262020 CTTCCTTCCACGAGGATCAG Chr9:21261950..21261969 59.8 55
upstream ENSMUSE00000584898 Chr9:21270026..21270175 TATGCGGAATTTTTGCACTG Chr9:21270028..21270047 59.7 40
upstream ENSMUSE00000584897 Chr9:21271976..21272179 CTCCCAGGCATCACTAAGGT Chr9:21271999..21272018 59.16 55
upstream ENSMUSE00000584896 Chr9:21275787..21275885 TCGGTGTCATCACCAAGCTA Chr9:21275799..21275818 60.26 50
upstream ENSMUSE00000584895 Chr9:21277057..21277217 AGAAAGACATCGAGGGCAAA Chr9:21277084..21277103 59.81 45
upstream ENSMUSE00000638336 Chr9:21278976..21279118 ACCAACCACATCCGAGAGTC Chr9:21278982..21279001 59.97 55
upstream ENSMUSE00000584893 Chr9:21280917..21281052 CCCGCATCAATCGTATCTTT Chr9:21281003..21281022 59.92 45
upstream ENSMUSE00000638335 Chr9:21282725..21282787 No primer for this exon
upstream ENSMUSE00000702744 Chr9:21282725..21282792 No primer for this exon
upstream ENSMUSE00000638334 Chr9:21283038..21283042 No primer for this exon
upstream ENSMUSE00000584892 Chr9:21283343..21283481 AATGCGTGGACCTGGTTATC Chr9:21283423..21283442 59.82 50
upstream ENSMUSE00000638333 Chr9:21284851..21284989 TGTGTTGACCTGGTGGTGTC Chr9:21284933..21284952 60.48 55
upstream ENSMUSE00000584891 Chr9:21285777..21285863 ACCTACATCAGGGAGCGAGA Chr9:21285825..21285844 59.83 55
upstream ENSMUSE00000584890 Chr9:21290095..21290165 No primer for this exon
upstream ENSMUSE00000584889 Chr9:21294187..21294238 ACAAGAAGAGGGCCATACCC Chr9:21294213..21294232 60.33 55
upstream ENSMUSE00000702763 Chr9:21295102..21295113 No primer for this exon
upstream ENSMUSE00000638331 Chr9:21298948..21299061 TTCGTGCTGACAGCTGAGTC Chr9:21299017..21299036 60.34 55

*** Putative Vector Insertion (Chr 9: 21299062 - 21303701) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000638330 Chr9:21303702..21303811 AAACACGTGCTTGTTGGACA Chr9:21303791..21303810 60.2 45
downstream ENSMUSE00000584885 Chr9:21304702..21304813 CTGGGAGTCACAAGCCAGTT Chr9:21304753..21304772 60.3 55
downstream ENSMUSE00000638329 Chr9:21307986..21308150 CACGGATGGACTTGTTGATG Chr9:21308112..21308131 59.96 50
downstream ENSMUSE00000584881 Chr9:21308874..21309106 CTCCTTCAGTGCGTGGTACA Chr9:21309011..21309030 59.9 55
downstream ENSMUSE00000584860 Chr9:21309908..21310159 GTGCACACTGGACACAGGTC Chr9:21309947..21309966 60.22 60
downstream ENSMUSE00000584869 Chr9:21310777..21311568 TCATACAGCCCAAACCACAA Chr9:21311419..21311438 59.96 45
downstream ENSMUSE00000584874 Chr9:21310780..21310846 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAGAGTGCTCTGTGGGGAAG Chr9:21302059..21302079 59.99 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GAGAGTGCTCTGTGGGGAAG Chr9:21302059..21302079 59.99 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033335