Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI7976
Trapped Gene
Riok1 (ENSMUSG00000021428)
Vector Insertion
Chr 13: 38132144 - 38134919
Public Clones RRI293 (baygenomics)
Private Clones OST390215 (lexicon)
Severity of mutation (?) Insertion after 16% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000117768 (Chr13:38131942..38132143 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000117768 (Chr13:38131942..38132143 +)
Downstram Exon
ENSMUSE00000117761 (Chr13:38134920..38135010 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000369398 Chr13:38129164..38129400 No primer for this exon
upstream ENSMUSE00000117768 Chr13:38131942..38132143 No primer for this exon

*** Putative Vector Insertion (Chr 13: 38132144 - 38134919) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000117761 Chr13:38134920..38135010 No primer for this exon
downstream ENSMUSE00000117767 Chr13:38135778..38135847 No primer for this exon
downstream ENSMUSE00000117770 Chr13:38137142..38137184 No primer for this exon
downstream ENSMUSE00000117759 Chr13:38139114..38139206 No primer for this exon
downstream ENSMUSE00000117754 Chr13:38140749..38140861 No primer for this exon
downstream ENSMUSE00000360014 Chr13:38140945..38141025 No primer for this exon
downstream ENSMUSE00000395103 Chr13:38142093..38142179 No primer for this exon
downstream ENSMUSE00000361699 Chr13:38142677..38142814 No primer for this exon
downstream ENSMUSE00000379963 Chr13:38144138..38144241 No primer for this exon
downstream ENSMUSE00000343740 Chr13:38144747..38144853 No primer for this exon
downstream ENSMUSE00000292210 Chr13:38148031..38148096 No primer for this exon
downstream ENSMUSE00000117763 Chr13:38149006..38149125 No primer for this exon
downstream ENSMUSE00000117765 Chr13:38150557..38150610 No primer for this exon
downstream ENSMUSE00000117756 Chr13:38151809..38151961 No primer for this exon
downstream ENSMUSE00000345118 Chr13:38152624..38153298 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
  No PCR available

Come back to gene ENSMUSG00000021428