Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI8265
Trapped Gene
Dzip3 (ENSMUSG00000064061)
Vector Insertion
Chr 16: 48931407 - 48933906
Public Clones RRF115 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 80% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000408300 (Chr16:48933907..48934008 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
CCTTGAGTACCTTGCCTCCA Chr16:48933946..48933965 60.25 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000408300 (Chr16:48933907..48934008 -)
Downstram Exon
ENSMUSE00000445206 (Chr16:48931281..48931406 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
CCTTGAGTACCTTGCCTCCA Chr16:48933946..48933965 60.25 55 AGAGATGACTCCAGGGCAAA Chr16:48931286..48931305 59.8 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000513113 Chr16:48993903..48994054 No primer for this exon
upstream ENSMUSE00000721921 Chr16:48993903..48994278 TCATTCTGTGGAGGGCTTCT Chr16:48994038..48994057 59.8 50
upstream ENSMUSE00000507298 Chr16:48984648..48984748 GGAACATTGTGCAACATGGA Chr16:48984675..48984694 60.38 45
upstream ENSMUSE00000699608 Chr16:48984648..48984679 No primer for this exon
upstream ENSMUSE00000303892 Chr16:48982173..48982245 GTGGAGGAACAGACCAAGGA Chr16:48982213..48982232 60.09 55
upstream ENSMUSE00000303882 Chr16:48981012..48981167 TCTTCCCAAAGGTGTGTTCC Chr16:48981062..48981081 59.94 50
upstream ENSMUSE00000303871 Chr16:48979659..48979775 CATAGCCAGACTGGGGAAGA Chr16:48979741..48979760 60.21 55
upstream ENSMUSE00000303860 Chr16:48977995..48978075 AGTGGCACTCACTGAAAGAGC Chr16:48978005..48978025 59.66 52.38
upstream ENSMUSE00000303851 Chr16:48975536..48975660 CGTGGTTTACTTCGATGTGC Chr16:48975545..48975564 59.2 50
upstream ENSMUSE00000303841 Chr16:48972335..48972449 No primer for this exon
upstream ENSMUSE00000303827 Chr16:48961221..48961340 TTGATTGTGAGCGTTCTTGTG Chr16:48961253..48961273 59.9 42.86
upstream ENSMUSE00000303813 Chr16:48959768..48959869 No primer for this exon
upstream ENSMUSE00000303802 Chr16:48958532..48958624 TGTGCCTGGAATTGTCAAAA Chr16:48958537..48958556 60.09 40
upstream ENSMUSE00000303789 Chr16:48957766..48957818 No primer for this exon
upstream ENSMUSE00000303779 Chr16:48953840..48953916 No primer for this exon
upstream ENSMUSE00000303772 Chr16:48951656..48952273 GATATTCTTCTGCGCCTTGG Chr16:48951793..48951812 59.81 50
upstream ENSMUSE00000303763 Chr16:48950114..48950146 No primer for this exon
upstream ENSMUSE00000488369 Chr16:48948520..48948689 TGAGTCCACCTGTGATGGAA Chr16:48948631..48948650 60.09 50
upstream ENSMUSE00000389007 Chr16:48947788..48947835 No primer for this exon
upstream ENSMUSE00000348060 Chr16:48945711..48945736 No primer for this exon
upstream ENSMUSE00000303739 Chr16:48944876..48945041 ACGACCAGCTGGAGACAGAA Chr16:48945002..48945021 61.01 55
upstream ENSMUSE00000129172 Chr16:48943570..48943665 GGCTTGCTCATCAACATCAG Chr16:48943597..48943616 59.4 50
upstream ENSMUSE00000340569 Chr16:48942189..48942316 ACAAGTGGCTTTTGGGATCA Chr16:48942202..48942221 60.49 45
upstream ENSMUSE00000558268 Chr16:48941765..48941833 CGCCAAATCCATGCTAAAGA Chr16:48941807..48941826 61.09 45
upstream ENSMUSE00000303707 Chr16:48938547..48938643 ACCAGCAGAGCCTTAACAGC Chr16:48938551..48938570 59.64 55
upstream ENSMUSE00000558266 Chr16:48937083..48937186 CACCTCGAGCAGACAGAAAA Chr16:48937128..48937147 59.16 50
upstream ENSMUSE00000558263 Chr16:48935612..48935699 GCGGATGTTCGAAACAAGAT Chr16:48935625..48935644 60.08 45
upstream ENSMUSE00000408300 Chr16:48933907..48934008 CCTTGAGTACCTTGCCTCCA Chr16:48933946..48933965 60.25 55

*** Putative Vector Insertion (Chr 16: 48931407 - 48933906) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000445206 Chr16:48931281..48931406 AGAGATGACTCCAGGGCAAA Chr16:48931286..48931305 59.8 50
downstream ENSMUSE00000129170 Chr16:48929729..48929868 GACTTGCACTCGGTGACACA Chr16:48929783..48929802 60.95 55
downstream ENSMUSE00000129167 Chr16:48928409..48928529 AGTCGAGACACGGACTTTCC Chr16:48928445..48928464 59.31 55
downstream ENSMUSE00000558262 Chr16:48927843..48927968 GGGAGCATTAGGCTGTGATG Chr16:48927875..48927894 60.62 55
downstream ENSMUSE00000558261 Chr16:48927655..48927756 TTTGTGAGCACAAGGCAAAA Chr16:48927645..48927664 60.42 40
downstream ENSMUSE00000558260 Chr16:48926105..48926215 CAGGGAATTCTTCTGGTTGC Chr16:48926115..48926134 59.67 50
downstream ENSMUSE00000699613 Chr16:48925992..48926215 CAGGGAATTCTTCTGGTTGC Chr16:48926115..48926134 59.67 50
downstream ENSMUSE00000716021 Chr16:48924345..48926215 GGTCAGCAAACTCTGCAACA Chr16:48924662..48924681 60.03 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCACCACCAAGTCCTGAGAT Chr16:48933909..48933929 59.96 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCACCACCAAGTCCTGAGAT Chr16:48933909..48933929 59.96 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000064061