Tools for the biologist enabling optimized use of gene trap clones
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About
UniTrap UNI864
Trapped Gene
Utp15 (
ENSMUSG00000041747
)
Vector Insertion
Chr 13: 99029422 - 99030753
Public Clones
CC0812 (sanger)
CB0188 (sanger)
Private Clones
not available
Severity of mutation
(?)
Insertion after 6% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000434206 (Chr13:99030754..99030925 -)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
TGGCCGGCTATAAACCTGTA
Chr13:99030823..99030842
60.47
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000434206 (Chr13:99030754..99030925 -)
Downstram Exon
ENSMUSE00000434193 (Chr13:99029329..99029421 -)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
TGGCCGGCTATAAACCTGTA
Chr13:99030823..99030842
60.47
50
GGACACTGCACCAAATTCCT
Chr13:99029364..99029383
59.97
50
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000434212
Chr13:99032830..99032947
TGCAGAGCGGTATGCTGTAG
Chr13:99032899..99032918
60.18
55
upstream
ENSMUSE00000434206
Chr13:99030754..99030925
TGGCCGGCTATAAACCTGTA
Chr13:99030823..99030842
60.47
50
*** Putative Vector Insertion (Chr 13: 99029422 - 99030753) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000434193
Chr13:99029329..99029421
GGACACTGCACCAAATTCCT
Chr13:99029364..99029383
59.97
50
downstream
ENSMUSE00000434187
Chr13:99029060..99029244
ACCATCCTGTCGAAAAGTCG
Chr13:99029130..99029149
60.11
50
downstream
ENSMUSE00000339862
Chr13:99027791..99027969
CGCACCTCACGTAATCAGAA
Chr13:99027808..99027827
59.86
50
downstream
ENSMUSE00000380856
Chr13:99027056..99027181
AGGGGAAAAGCAGGACACTC
Chr13:99027058..99027077
60.63
55
downstream
ENSMUSE00000337545
Chr13:99024874..99025009
CCAGTGAGCCGGAGAGTAAC
Chr13:99024856..99024875
59.87
60
downstream
ENSMUSE00000296346
Chr13:99023599..99023683
TGAAGCTGCGTAGTCAAAGC
Chr13:99023598..99023617
59.37
50
downstream
ENSMUSE00000296336
Chr13:99022700..99022849
TTGCTTCAGACTTCCGGTGT
Chr13:99022755..99022774
60.83
50
downstream
ENSMUSE00000296326
Chr13:99021884..99021985
TCTTTGCTGGCCTACTGACC
Chr13:99021933..99021952
60.4
55
downstream
ENSMUSE00000296319
Chr13:99020740..99020873
TTAAGGACCCGGGTAACCTC
Chr13:99020730..99020749
60.18
55
downstream
ENSMUSE00000296309
Chr13:99020527..99020585
GAACCTCGGCTGAGACAGAT
Chr13:99020542..99020561
59.41
55
downstream
ENSMUSE00000480312
Chr13:99016800..99019331
GAAGTGACCAGCGAGAGGAC
Chr13:99018627..99018646
59.99
60
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TGAGAGGAAATGAGGGACGA
Chr13:99030724..99030744
60.74
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
TGAGAGGAAATGAGGGACGA
Chr13:99030724..99030744
60.74
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000041747