Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI8660
Trapped Gene
Zfp619 (ENSMUSG00000068959)
Vector Insertion
Chr 7: 46773235 - 46789162
Public Clones RRK232 (baygenomics)
Private Clones OST434755 (lexicon) OST354432 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000675249 (Chr7:46773136..46773234 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
AGTGCAGTCGAGATCGGAAG Chr7:46773205..46773224 60.56 55 TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000675249 (Chr7:46773136..46773234 +)
Downstram Exon
ENSMUSE00000567566 (Chr7:46789163..46789289 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
AGTGCAGTCGAGATCGGAAG Chr7:46773205..46773224 60.56 55 GTCCACTCTTCCTGGGTGAA Chr7:46789218..46789237 60.09 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000675249 Chr7:46773136..46773234 AGTGCAGTCGAGATCGGAAG Chr7:46773205..46773224 60.56 55

*** Putative Vector Insertion (Chr 7: 46773235 - 46789162) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000567566 Chr7:46789163..46789289 GTCCACTCTTCCTGGGTGAA Chr7:46789218..46789237 60.09 55
downstream ENSMUSE00000198732 Chr7:46789485..46789545 TTCCCAGGCTGTTTATACTGG Chr7:46789538..46789558 59.1 47.62
downstream ENSMUSE00000493432 Chr7:46790109..46793403 GTTGAAGGGAACTCGAGCAG Chr7:46791883..46791902 59.99 55
downstream ENSMUSE00000675247 Chr7:46790109..46795790 AGTTGAAGGGAACTCGAGCA Chr7:46791884..46791903 59.99 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGGGAGAGTGCAGTCGAGAT Chr7:46782200..46782220 61.76 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 GGGGAGAGTGCAGTCGAGAT Chr7:46782200..46782220 61.76 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000068959