Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI8731
Trapped Gene
Med13l (ENSMUSG00000018076)
Vector Insertion
Chr 5: 119010867 - 119043341
Public Clones (sanger) (sanger) XN687 (baygenomics) IST14466E4 (tigm) IST10126F12 (tigm)
Private Clones OST311787 (lexicon) OST249482 (lexicon) OST192247 (lexicon) OST188908 (lexicon)
OST187190 (lexicon) OST138980 (lexicon) OST126660 (lexicon)
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000538988 (Chr5:119010752..119010866 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000538988 (Chr5:119010752..119010866 +)
Downstram Exon
ENSMUSE00000352988 (Chr5:119043342..119043579 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000538988 Chr5:119010752..119010866 No primer for this exon

*** Putative Vector Insertion (Chr 5: 119010867 - 119043341) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000352988 Chr5:119043342..119043579 No primer for this exon
downstream ENSMUSE00000290060 Chr5:119121022..119121106 No primer for this exon
downstream ENSMUSE00000290053 Chr5:119130897..119130980 No primer for this exon
downstream ENSMUSE00000483487 Chr5:119168492..119168637 No primer for this exon
downstream ENSMUSE00000484364 Chr5:119171384..119171578 No primer for this exon
downstream ENSMUSE00000466399 Chr5:119171774..119171947 No primer for this exon
downstream ENSMUSE00000467271 Chr5:119173997..119174162 No primer for this exon
downstream ENSMUSE00000498273 Chr5:119176270..119176374 No primer for this exon
downstream ENSMUSE00000495429 Chr5:119178157..119178888 No primer for this exon
downstream ENSMUSE00000496208 Chr5:119179779..119180004 No primer for this exon
downstream ENSMUSE00000493352 Chr5:119180921..119181026 No primer for this exon
downstream ENSMUSE00000494634 Chr5:119181337..119181461 No primer for this exon
downstream ENSMUSE00000491693 Chr5:119183968..119184067 No primer for this exon
downstream ENSMUSE00000492550 Chr5:119188324..119188544 No primer for this exon
downstream ENSMUSE00000505150 Chr5:119188804..119189009 No primer for this exon
downstream ENSMUSE00000289867 Chr5:119191835..119192784 No primer for this exon
downstream ENSMUSE00000289947 Chr5:119195018..119195197 No primer for this exon
downstream ENSMUSE00000290134 Chr5:119195651..119195874 No primer for this exon
downstream ENSMUSE00000188166 Chr5:119197328..119197520 No primer for this exon
downstream ENSMUSE00000289938 Chr5:119198573..119198990 No primer for this exon
downstream ENSMUSE00000289929 Chr5:119199618..119199837 No primer for this exon
downstream ENSMUSE00000188159 Chr5:119200855..119201043 No primer for this exon
downstream ENSMUSE00000188163 Chr5:119201540..119201763 No primer for this exon
downstream ENSMUSE00000290099 Chr5:119201849..119202018 No primer for this exon
downstream ENSMUSE00000188171 Chr5:119204272..119204430 No primer for this exon
downstream ENSMUSE00000188165 Chr5:119205548..119205724 No primer for this exon
downstream ENSMUSE00000289863 Chr5:119207086..119207243 No primer for this exon
downstream ENSMUSE00000289858 Chr5:119209111..119209272 No primer for this exon
downstream ENSMUSE00000650454 Chr5:119211295..119211407 No primer for this exon
downstream ENSMUSE00000650453 Chr5:119212710..119212989 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 CCTCTTCTCGCTGGTGAGTG Chr5:119034855..119034875 61.14 60 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CCTCTTCTCGCTGGTGAGTG Chr5:119034855..119034875 61.14 60 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000018076