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About
UniTrap UNI8799
Trapped Gene
Utx (
ENSMUSG00000037369
)
Vector Insertion
Chr X: 17776393 - 17785861
Public Clones
TEA047 (baygenomics)
RRA094 (baygenomics)
Private Clones
not available
Severity of mutation
(?)
Insertion after 8% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000557269 (ChrX:17776284..17776392 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
TGTTCGCTGCTACGAATCTC
ChrX:17776286..17776305
59.18
50
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000557269 (ChrX:17776284..17776392 +)
Downstram Exon
ENSMUSE00000557267 (ChrX:17785862..17785911 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
TGTTCGCTGCTACGAATCTC
ChrX:17776286..17776305
59.18
50
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000702729
ChrX:17739701..17740298
TACCGCGTTAGGATTTTTCG
ChrX:17739973..17739992
60.09
45
upstream
ENSMUSE00000457469
ChrX:17739793..17740298
TACCGCGTTAGGATTTTTCG
ChrX:17739973..17739992
60.09
45
upstream
ENSMUSE00000503440
ChrX:17740510..17740573
No primer for this exon
upstream
ENSMUSE00000557269
ChrX:17776284..17776392
TGTTCGCTGCTACGAATCTC
ChrX:17776286..17776305
59.18
50
*** Putative Vector Insertion (Chr X: 17776393 - 17785861) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000557267
ChrX:17785862..17785911
No primer for this exon
downstream
ENSMUSE00000557262
ChrX:17794064..17794122
No primer for this exon
downstream
ENSMUSE00000557258
ChrX:17799883..17800003
ATTTCCTTGGCTCGACAAAA
ChrX:17799948..17799967
59.69
40
downstream
ENSMUSE00000457430
ChrX:17810986..17811040
TTGGACAAAGTGCAGGGATT
ChrX:17811035..17811054
60.49
45
downstream
ENSMUSE00000557250
ChrX:17813787..17813821
GTGGGCAATGTGAAACTGAA
ChrX:17813809..17813828
59.55
45
downstream
ENSMUSE00000510606
ChrX:17815967..17816060
TCTGTCTGCAAAAGTTGCTCA
ChrX:17816018..17816038
59.77
42.86
downstream
ENSMUSE00000557245
ChrX:17818828..17818954
TTGGATCTGCTTCCAAGGAC
ChrX:17818926..17818945
60.19
50
downstream
ENSMUSE00000557241
ChrX:17823092..17823190
GCATCCTGAACTTTCCCAAT
ChrX:17823127..17823146
58.98
45
downstream
ENSMUSE00000557238
ChrX:17823323..17823542
TAATTCGTGCTGCAAGTCCA
ChrX:17823534..17823553
60.4
45
downstream
ENSMUSE00000557235
ChrX:17824101..17824235
GCTCCATGCCTCCTCAATAC
ChrX:17824181..17824200
59.66
55
downstream
ENSMUSE00000327156
ChrX:17825396..17825485
TGCCACCACTCCAATTATCA
ChrX:17825426..17825445
59.92
45
downstream
ENSMUSE00000327149
ChrX:17826879..17826980
CTTTCCAGCTGTTCCAGCAT
ChrX:17826955..17826974
60.4
50
downstream
ENSMUSE00000327142
ChrX:17827833..17828228
CAGGTAAGGCACGTTTCCAT
ChrX:17828138..17828157
59.99
50
downstream
ENSMUSE00000327138
ChrX:17831130..17831908
CACCGTCAATGTGTTTCCTG
ChrX:17831390..17831409
60
50
downstream
ENSMUSE00000557228
ChrX:17834962..17835091
GTCTTGGAGGCGGACATTTA
ChrX:17835029..17835048
60.07
50
downstream
ENSMUSE00000557225
ChrX:17838649..17838754
AACAGGGTTGTTTGGGTTTG
ChrX:17838720..17838739
59.73
45
downstream
ENSMUSE00000327122
ChrX:17839356..17839561
GGTTCCAGTAGGGTCCCAAT
ChrX:17839471..17839490
60.05
55
downstream
ENSMUSE00000327120
ChrX:17841074..17841138
No primer for this exon
downstream
ENSMUSE00000557221
ChrX:17841212..17841286
TGTTGGTCCCAAACTTAATGG
ChrX:17841266..17841286
59.71
42.86
downstream
ENSMUSE00000557220
ChrX:17841688..17841836
TCTCACGAAGGCAGGAAGTT
ChrX:17841739..17841758
59.99
50
downstream
ENSMUSE00000557215
ChrX:17846274..17846388
AAACACCCCAGTAGCCTTCA
ChrX:17846372..17846391
59.59
50
downstream
ENSMUSE00000557211
ChrX:17848971..17849158
TCCAGACCAGATCTCCAGGT
ChrX:17849083..17849102
59.64
55
downstream
ENSMUSE00000557208
ChrX:17850074..17850215
CGTGCCATATTCCAGGAAAG
ChrX:17850176..17850195
60.46
50
downstream
ENSMUSE00000327083
ChrX:17852161..17852287
CCCGTGCCATATGATCTCTT
ChrX:17852248..17852267
59.92
50
downstream
ENSMUSE00000354140
ChrX:17854502..17854689
TTTTTCGTGCACAATCTTGG
ChrX:17854592..17854611
59.71
40
downstream
ENSMUSE00000557200
ChrX:17854502..17854672
TTTTTCGTGCACAATCTTGG
ChrX:17854592..17854611
59.71
40
downstream
ENSMUSE00000470278
ChrX:17855752..17856478
AGATGAGGCGGATGGTAATG
ChrX:17855781..17855800
59.92
50
downstream
ENSMUSE00000557198
ChrX:17855752..17857062
AGATGAGGCGGATGGTAATG
ChrX:17855781..17855800
59.92
50
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
TGTCAATTAGGTCACTTCAACCTC
ChrX:17785348..17785372
59.56
41.67
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CTAAACGTGACTGGGAAAACC
ChrX:17785439..17785460
58.61
47.62
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000037369