Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI8827
Trapped Gene
Usp37 (ENSMUSG00000033364)
Vector Insertion
Chr 1: 74491103 - 74497068
Public Clones TEA217 (baygenomics)
Private Clones not available
Severity of mutation (?) Insertion after 86% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000271665 (Chr1:74497069..74497129 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
TGACGACCTCAAAAGGGCTA Chr1:74497087..74497106 60.77 50 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000271665 (Chr1:74497069..74497129 -)
Downstram Exon
ENSMUSE00000271657 (Chr1:74490987..74491102 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
TGACGACCTCAAAAGGGCTA Chr1:74497087..74497106 60.77 50 GCTCCTCAGCTTCAGCTTCT Chr1:74490982..74491001 59.07 55

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000436270 Chr1:74590749..74590860 AGGATGGTTACAACGGATGC Chr1:74590827..74590846 59.82 50
upstream ENSMUSE00000436249 Chr1:74577637..74577686 AGGTTCTGCCTGTGCAAGAG Chr1:74577649..74577668 60.59 55
upstream ENSMUSE00000435843 Chr1:74546530..74546674 ACCACTGGTAGAGCCGAAGA Chr1:74546632..74546651 59.87 55
upstream ENSMUSE00000347338 Chr1:74542204..74542427 CAGCCTGCTCGTTCACTACA Chr1:74542233..74542252 60.2 55
upstream ENSMUSE00000393309 Chr1:74539479..74539650 GCCGTCCATCAAAACAGACT Chr1:74539487..74539506 60.12 50
upstream ENSMUSE00000271774 Chr1:74536664..74536764 AACTATTCTGGGCAGCAGGA Chr1:74536711..74536730 59.84 50
upstream ENSMUSE00000271763 Chr1:74533193..74533371 TGACGCTGACATCAACACCT Chr1:74533219..74533238 60.32 50
upstream ENSMUSE00000271752 Chr1:74532555..74532629 No primer for this exon
upstream ENSMUSE00000271745 Chr1:74526226..74526323 AGCAGCTGAGTTTGAAACAGG Chr1:74526247..74526267 59.67 47.62
upstream ENSMUSE00000271737 Chr1:74524621..74524705 GAGCAGAGCTGGATCAAAGG Chr1:74524652..74524671 60.1 55
upstream ENSMUSE00000271730 Chr1:74521560..74521718 GACCTAGAGTGCCCCTTTCC Chr1:74521583..74521602 60.07 60
upstream ENSMUSE00000271725 Chr1:74518129..74518260 TCCAACTTGGGAAATACATGC Chr1:74518236..74518256 59.81 42.86
upstream ENSMUSE00000271718 Chr1:74517870..74517993 ACGCTTTGCAAACCTGCTTA Chr1:74517974..74517993 60.93 45
upstream ENSMUSE00000271712 Chr1:74517111..74517301 AGTGTTCACGTGCCCTGTTA Chr1:74517157..74517176 59.21 50
upstream ENSMUSE00000271706 Chr1:74514841..74514958 CCTCCTCGTTCAATCCAAGA Chr1:74514863..74514882 60.19 50
upstream ENSMUSE00000271698 Chr1:74513489..74513568 TGCTCTTGTCAGGCACAAAT Chr1:74513505..74513524 59.44 45
upstream ENSMUSE00000271692 Chr1:74508140..74508304 AACAAGCTCGGACAGCAAGT Chr1:74508230..74508249 60.06 50
upstream ENSMUSE00000271689 Chr1:74506489..74506554 TGAGAGCCTGTCAAATGGTG Chr1:74506524..74506543 59.83 50
upstream ENSMUSE00000271685 Chr1:74502653..74502794 ATCCAAGAGCTCCGTGACAT Chr1:74502760..74502779 59.69 50
upstream ENSMUSE00000271675 Chr1:74500402..74500657 GATAAGCCAACCAGCAGTCC Chr1:74500494..74500513 59.7 55
upstream ENSMUSE00000271669 Chr1:74499261..74499427 TGATTGGCTCCAGCAGTATG Chr1:74499326..74499345 59.82 50
upstream ENSMUSE00000271665 Chr1:74497069..74497129 TGACGACCTCAAAAGGGCTA Chr1:74497087..74497106 60.77 50

*** Putative Vector Insertion (Chr 1: 74491103 - 74497068) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000271657 Chr1:74490987..74491102 GCTCCTCAGCTTCAGCTTCT Chr1:74490982..74491001 59.07 55
downstream ENSMUSE00000271653 Chr1:74488106..74488175 TGAGCCGATAGGAATGAGGA Chr1:74488123..74488142 60.7 50
downstream ENSMUSE00000271649 Chr1:74486534..74486672 CTGCGATCTCGATCACTCTG Chr1:74486539..74486558 59.68 55
downstream ENSMUSE00000356685 Chr1:74485686..74486052 AAGGAAGCGGCACTCTTGTA Chr1:74485712..74485731 60.01 50

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 TGACGACCTCAAAAGGGCTA Chr1:74494085..74494105 60.77 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTTCAGCGTGACTGGGAAAA Chr1:74494087..74494107 61.34 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000033364