Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI9027
Trapped Gene
Cdh1 (ENSMUSG00000000303)
Vector Insertion
Chr 8: 109128308 - 109172900
Public Clones (sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) (sanger) (sanger)
(sanger) (sanger) (sanger) (sanger) RST560 (baygenomics) XST130 (baygenomics)
GST159 (baygenomics) RST513 (baygenomics) PST035 (baygenomics) PST103 (baygenomics)
GST193 (baygenomics) GST073 (baygenomics) XK406 (baygenomics) PST024 (baygenomics)
KST259 (baygenomics) RST385 (baygenomics) PST037 (baygenomics) PST113 (baygenomics)
XST071 (baygenomics) GST126 (baygenomics) RST465 (baygenomics) PST027 (baygenomics)
KST281 (baygenomics) RST102 (baygenomics) PST058 (baygenomics) G051A11 (ggtc)
G038B12 (ggtc) G029E05 (ggtc) G051G04 (ggtc) E325H01 (ggtc) E324G06 (ggtc)
E323H06 (ggtc) E323B01 (ggtc) D152A09 (ggtc) D099F12 (ggtc) D050D07 (ggtc)
(ggtc) G054C10 (ggtc) G075F04 (ggtc) G054F04 (ggtc) E325B09 (ggtc)
E324F04 (ggtc) E323G11 (ggtc) E323A03 (ggtc) D142A09 (ggtc) D099F01 (ggtc)
(ggtc) G057A06 (ggtc) G039G08 (ggtc) G011B06 (ggtc) G004B10 (ggtc)
E325A04 (ggtc) E325A12 (ggtc) E324A07 (ggtc) E323F07 (ggtc) D162E04 (ggtc)
D124H08 (ggtc) D058E04 (ggtc) (ggtc) G045C12 (ggtc) G080G02 (ggtc)
G069A08 (ggtc) G051F11 (ggtc) E325D04 (ggtc) E324G06 (ggtc) E323H06 (ggtc)
E323B01 (ggtc) D152A09 (ggtc) D099F12 (ggtc) (ggtc) G057D12 (ggtc)
G075B03 (ggtc) G017F11 (ggtc) E325B08 (ggtc) E324D08 (ggtc) E323G11 (ggtc)
D164F10 (ggtc) D139D03 (ggtc) D098B09 (ggtc) (ggtc) G051E03 (ggtc)
G085G09 (ggtc) G047D09 (ggtc) G051B06 (ggtc) E325H01 (ggtc) E325A04 (ggtc)
E324A07 (ggtc) E323F07 (ggtc) D160E04 (ggtc) D112H05 (ggtc) D050D07 (ggtc)
(ggtc) G047E02 (ggtc) G079E08 (ggtc) G056A06 (ggtc) E325C12 (ggtc)
E324F04 (ggtc) E323H04 (ggtc) E323A03 (ggtc) D142A09 (ggtc) D099F01 (ggtc)
5SE325D04 (ggtc) (ggtc) P069E03 (ggtc) G085H09 (ggtc) G070A12 (ggtc)
G003D05 (ggtc) E325B08 (ggtc) E325A12 (ggtc) E324D08 (ggtc) E323G05 (ggtc)
D164F10 (ggtc) D139D03 (ggtc) D059F09 (ggtc) (ggtc) CMHD-GT_460G11-3 (cmhd)
CMHD-GT_522F7-5S (cmhd) CMHD-GT_499G9-3 (cmhd) CMHD-GT_411D3-3 (cmhd) CMHD-GT_430E11-3 (cmhd)
CMHD-GT_428B1-3 (cmhd) CMHD-GT_522F7-3 (cmhd) CMHD-GT_421C10-3 (cmhd) CMHD-GT_320H8-3 (cmhd)
CMHD-GT_264G05-3 (cmhd) CMHD-GT_523D5-3 (cmhd) PST11726-NL (escells) IST14390D3 (tigm)
IST14724B5 (tigm) IST14022C7 (tigm) IST12511D8 (tigm) IST13698H3 (tigm)
IST13281A10 (tigm) IST14431C10 (tigm) IST14925A10 (tigm) IST12372A10HMF1 (tigm)
IST14266G5 (tigm) IST14098F9 (tigm) IST12232B12 (tigm) IST10786F6 (tigm)
IST11876H2 (tigm) IST14116H3 (tigm) IST13117E6 (tigm) IST14698B4 (tigm)
IST14181G8 (tigm) IST13928H6 (tigm) IST14099B8 (tigm) IST12330B1 (tigm)
IST10921D11 (tigm) IST14758F2 (tigm) IST11522C8 (tigm) IST14652A12 (tigm)
IST12677D2 (tigm) IST10786F6 (tigm) IST14240D3 (tigm) IST13928H6 (tigm)
IST12498H3 (tigm) IST12788F10 (tigm) IST11020C3 (tigm) IST12276G2 (tigm)
IST14706C4 (tigm) IST10374G7 (tigm) IST13439G9 (tigm) IST14795G11 (tigm)
IST14719H4 (tigm) IST13954H10 (tigm) IST10423H6 (tigm) IST12498H3 (tigm)
IST14099B10 (tigm) IST12232C12 (tigm) IST10479G6 (tigm) IST12330B1 (tigm)
IST10892D9 (tigm) IST14022C7 (tigm) IST11782A12 (tigm) IST11446B8 (tigm)
IST13334D12 (tigm) IST11020C3 (tigm) IST14098F9 (tigm) IST12677D2 (tigm)
IST10179C7 (tigm) IST13762C3 (tigm) IST10179C7 (tigm) IST12476B1 (tigm)
IST11782A12 (tigm) IST14035C3 (tigm) IST10181H8 (tigm) IST13899A3 (tigm)
IST12511D8 (tigm) IST14753A9 (tigm)
Private Clones OST445171 (lexicon) OST422382 (lexicon) OST420834 (lexicon) OST257927 (lexicon)
OST124988 (lexicon) OST77187 (lexicon) OST43749 (lexicon) OST38921 (lexicon)
OST38564 (lexicon)
Severity of mutation (?) Insertion after 6% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000214612 (Chr8:109128187..109128307 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000214612 (Chr8:109128187..109128307 +)
Downstram Exon
ENSMUSE00000214610 (Chr8:109172901..109173124 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000334955 Chr8:109127268..109127442 No primer for this exon
upstream ENSMUSE00000214612 Chr8:109128187..109128307 No primer for this exon

*** Putative Vector Insertion (Chr 8: 109128308 - 109172900) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000214610 Chr8:109172901..109173124 No primer for this exon
downstream ENSMUSE00000214615 Chr8:109177407..109177550 No primer for this exon
downstream ENSMUSE00000214606 Chr8:109177664..109177819 No primer for this exon
downstream ENSMUSE00000214613 Chr8:109180724..109180868 No primer for this exon
downstream ENSMUSE00000214611 Chr8:109181640..109181815 No primer for this exon
downstream ENSMUSE00000214609 Chr8:109182091..109182219 No primer for this exon
downstream ENSMUSE00000214607 Chr8:109183490..109183672 No primer for this exon
downstream ENSMUSE00000214608 Chr8:109184696..109184940 No primer for this exon
downstream ENSMUSE00000330782 Chr8:109185785..109185930 No primer for this exon
downstream ENSMUSE00000330746 Chr8:109187647..109187871 No primer for this exon
downstream ENSMUSE00000330716 Chr8:109188096..109188323 No primer for this exon
downstream ENSMUSE00000330686 Chr8:109189294..109189424 No primer for this exon
downstream ENSMUSE00000330659 Chr8:109190087..109190230 No primer for this exon
downstream ENSMUSE00000469626 Chr8:109192306..109194146 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GCTGCCTGGTTTTGTGTTAGA Chr8:109152304..109152325 60.3 47.62 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ACTTTCCATTCCCGGACAAG Chr8:109152272..109152292 61.24 50 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000000303