Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI9493
Trapped Gene
Lama5 (ENSMUSG00000015647)
Vector Insertion
Chr 2: 179943077 - 179955930
Public Clones RST053 (baygenomics) LST031 (baygenomics) P083C03 (ggtc) P077G01 (ggtc)
P080E10 (ggtc) P077C03 (ggtc) P085H04 (ggtc) P083C06 (ggtc) P080D11 (ggtc)
P076G11 (ggtc) P077C06 (ggtc) P084H07 (ggtc) G069F11 (ggtc)
Private Clones not available
Severity of mutation (?) Insertion after 0% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000678447 (Chr2:179955931..179956083 -)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000678447 (Chr2:179955931..179956083 -)
Downstram Exon
ENSMUSE00000226030 (Chr2:179942959..179943076 -)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000717239 Chr2:179960196..179960564 No primer for this exon
upstream ENSMUSE00000719761 Chr2:179960196..179960564 No primer for this exon
upstream ENSMUSE00000226035 Chr2:179955931..179956083 No primer for this exon
upstream ENSMUSE00000678447 Chr2:179955931..179956083 No primer for this exon

*** Putative Vector Insertion (Chr 2: 179943077 - 179955930) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000226030 Chr2:179942959..179943076 No primer for this exon
downstream ENSMUSE00000678446 Chr2:179942959..179943076 No primer for this exon
downstream ENSMUSE00000170633 Chr2:179941892..179942010 No primer for this exon
downstream ENSMUSE00000678445 Chr2:179941892..179942010 No primer for this exon
downstream ENSMUSE00000170616 Chr2:179941638..179941808 No primer for this exon
downstream ENSMUSE00000678444 Chr2:179941638..179941808 No primer for this exon
downstream ENSMUSE00000170586 Chr2:179941430..179941527 No primer for this exon
downstream ENSMUSE00000678443 Chr2:179941430..179941527 No primer for this exon
downstream ENSMUSE00000170623 Chr2:179937097..179937212 No primer for this exon
downstream ENSMUSE00000678442 Chr2:179937097..179937212 No primer for this exon
downstream ENSMUSE00000170595 Chr2:179936866..179936984 No primer for this exon
downstream ENSMUSE00000678441 Chr2:179936866..179936984 No primer for this exon
downstream ENSMUSE00000170570 Chr2:179936684..179936774 No primer for this exon
downstream ENSMUSE00000678440 Chr2:179936684..179936774 No primer for this exon
downstream ENSMUSE00000170596 Chr2:179936426..179936560 No primer for this exon
downstream ENSMUSE00000678439 Chr2:179936426..179936560 No primer for this exon
downstream ENSMUSE00000170634 Chr2:179936163..179936225 No primer for this exon
downstream ENSMUSE00000678438 Chr2:179936163..179936225 No primer for this exon
downstream ENSMUSE00000170620 Chr2:179933855..179933995 No primer for this exon
downstream ENSMUSE00000678437 Chr2:179933855..179933995 No primer for this exon
downstream ENSMUSE00000170589 Chr2:179933635..179933772 No primer for this exon
downstream ENSMUSE00000678436 Chr2:179933635..179933772 No primer for this exon
downstream ENSMUSE00000170566 Chr2:179933401..179933535 No primer for this exon
downstream ENSMUSE00000678435 Chr2:179933401..179933535 No primer for this exon
downstream ENSMUSE00000170593 Chr2:179933180..179933314 No primer for this exon
downstream ENSMUSE00000678434 Chr2:179933180..179933314 No primer for this exon
downstream ENSMUSE00000170590 Chr2:179932966..179933103 No primer for this exon
downstream ENSMUSE00000678433 Chr2:179932966..179933103 No primer for this exon
downstream ENSMUSE00000170619 Chr2:179932422..179932474 No primer for this exon
downstream ENSMUSE00000678432 Chr2:179932422..179932474 No primer for this exon
downstream ENSMUSE00000170568 Chr2:179932078..179932183 No primer for this exon
downstream ENSMUSE00000678431 Chr2:179932078..179932183 No primer for this exon
downstream ENSMUSE00000170571 Chr2:179931931..179931980 No primer for this exon
downstream ENSMUSE00000678430 Chr2:179931931..179931980 No primer for this exon
downstream ENSMUSE00000170602 Chr2:179931741..179931846 No primer for this exon
downstream ENSMUSE00000678429 Chr2:179931741..179931846 No primer for this exon
downstream ENSMUSE00000170588 Chr2:179931189..179931288 No primer for this exon
downstream ENSMUSE00000678428 Chr2:179931189..179931288 No primer for this exon
downstream ENSMUSE00000170584 Chr2:179930873..179931029 No primer for this exon
downstream ENSMUSE00000678426 Chr2:179930873..179931029 No primer for this exon
downstream ENSMUSE00000170607 Chr2:179930586..179930724 No primer for this exon
downstream ENSMUSE00000678425 Chr2:179930586..179930724 No primer for this exon
downstream ENSMUSE00000170574 Chr2:179930260..179930402 No primer for this exon
downstream ENSMUSE00000678423 Chr2:179930260..179930402 No primer for this exon
downstream ENSMUSE00000170599 Chr2:179930077..179930183 No primer for this exon
downstream ENSMUSE00000678421 Chr2:179930077..179930183 No primer for this exon
downstream ENSMUSE00000170601 Chr2:179929851..179930004 No primer for this exon
downstream ENSMUSE00000678419 Chr2:179929851..179930004 No primer for this exon
downstream ENSMUSE00000170576 Chr2:179929380..179929540 No primer for this exon
downstream ENSMUSE00000678417 Chr2:179929380..179929540 No primer for this exon
downstream ENSMUSE00000170626 Chr2:179929195..179929303 No primer for this exon
downstream ENSMUSE00000678415 Chr2:179929195..179929303 No primer for this exon
downstream ENSMUSE00000170622 Chr2:179928680..179928777 No primer for this exon
downstream ENSMUSE00000678414 Chr2:179928680..179928777 No primer for this exon
downstream ENSMUSE00000170624 Chr2:179928397..179928598 No primer for this exon
downstream ENSMUSE00000678413 Chr2:179928397..179928598 No primer for this exon
downstream ENSMUSE00000170621 Chr2:179928109..179928235 No primer for this exon
downstream ENSMUSE00000678412 Chr2:179928109..179928235 No primer for this exon
downstream ENSMUSE00000170611 Chr2:179927568..179927707 No primer for this exon
downstream ENSMUSE00000678410 Chr2:179927568..179927707 No primer for this exon
downstream ENSMUSE00000170578 Chr2:179927094..179927209 No primer for this exon
downstream ENSMUSE00000678409 Chr2:179927094..179927209 No primer for this exon
downstream ENSMUSE00000170615 Chr2:179926799..179927010 No primer for this exon
downstream ENSMUSE00000678407 Chr2:179926799..179927010 No primer for this exon
downstream ENSMUSE00000225872 Chr2:179926256..179926460 No primer for this exon
downstream ENSMUSE00000678406 Chr2:179926256..179926460 No primer for this exon
downstream ENSMUSE00000170629 Chr2:179925933..179926077 No primer for this exon
downstream ENSMUSE00000678404 Chr2:179925933..179926077 No primer for this exon
downstream ENSMUSE00000170627 Chr2:179925610..179925750 No primer for this exon
downstream ENSMUSE00000678403 Chr2:179925610..179925750 No primer for this exon
downstream ENSMUSE00000170618 Chr2:179925345..179925506 No primer for this exon
downstream ENSMUSE00000678402 Chr2:179925345..179925506 No primer for this exon
downstream ENSMUSE00000170637 Chr2:179925035..179925136 No primer for this exon
downstream ENSMUSE00000678401 Chr2:179925035..179925136 No primer for this exon
downstream ENSMUSE00000170598 Chr2:179924865..179924948 No primer for this exon
downstream ENSMUSE00000591578 Chr2:179924865..179924948 No primer for this exon
downstream ENSMUSE00000170635 Chr2:179924038..179924268 No primer for this exon
downstream ENSMUSE00000170617 Chr2:179923272..179923385 No primer for this exon
downstream ENSMUSE00000170594 Chr2:179922970..179923097 No primer for this exon
downstream ENSMUSE00000591577 Chr2:179922613..179922644 No primer for this exon
downstream ENSMUSE00000170585 Chr2:179922564..179922644 No primer for this exon
downstream ENSMUSE00000170632 Chr2:179922251..179922465 No primer for this exon
downstream ENSMUSE00000170636 Chr2:179921881..179921981 No primer for this exon
downstream ENSMUSE00000678400 Chr2:179921881..179921897 No primer for this exon
downstream ENSMUSE00000170638 Chr2:179921506..179921686 No primer for this exon
downstream ENSMUSE00000170610 Chr2:179921267..179921416 No primer for this exon
downstream ENSMUSE00000170582 Chr2:179920519..179920670 No primer for this exon
downstream ENSMUSE00000170606 Chr2:179920328..179920438 No primer for this exon
downstream ENSMUSE00000678399 Chr2:179920324..179920438 No primer for this exon
downstream ENSMUSE00000170580 Chr2:179920034..179920145 No primer for this exon
downstream ENSMUSE00000170569 Chr2:179919106..179919276 No primer for this exon
downstream ENSMUSE00000225769 Chr2:179918723..179918919 No primer for this exon
downstream ENSMUSE00000225760 Chr2:179918323..179918439 No primer for this exon
downstream ENSMUSE00000225754 Chr2:179918025..179918179 No primer for this exon
downstream ENSMUSE00000225747 Chr2:179917669..179917810 No primer for this exon
downstream ENSMUSE00000392335 Chr2:179917472..179917574 No primer for this exon
downstream ENSMUSE00000348555 Chr2:179917063..179917173 No primer for this exon
downstream ENSMUSE00000678398 Chr2:179917059..179917173 No primer for this exon
downstream ENSMUSE00000384695 Chr2:179916277..179916456 No primer for this exon
downstream ENSMUSE00000399794 Chr2:179916055..179916203 No primer for this exon
downstream ENSMUSE00000546393 Chr2:179916055..179916142 No primer for this exon
downstream ENSMUSE00000170613 Chr2:179915798..179915974 No primer for this exon
downstream ENSMUSE00000170583 Chr2:179915547..179915683 No primer for this exon
downstream ENSMUSE00000170572 Chr2:179915314..179915467 No primer for this exon
downstream ENSMUSE00000170592 Chr2:179915015..179915148 No primer for this exon
downstream ENSMUSE00000170579 Chr2:179914779..179914941 No primer for this exon
downstream ENSMUSE00000170575 Chr2:179914541..179914663 No primer for this exon
downstream ENSMUSE00000170581 Chr2:179914311..179914459 No primer for this exon
downstream ENSMUSE00000170625 Chr2:179913957..179914233 No primer for this exon
downstream ENSMUSE00000170639 Chr2:179913715..179913845 No primer for this exon
downstream ENSMUSE00000170608 Chr2:179913426..179913596 No primer for this exon
downstream ENSMUSE00000170604 Chr2:179913220..179913334 No primer for this exon
downstream ENSMUSE00000170597 Chr2:179912982..179913127 No primer for this exon
downstream ENSMUSE00000170631 Chr2:179912714..179912909 No primer for this exon
downstream ENSMUSE00000170567 Chr2:179912440..179912604 No primer for this exon
downstream ENSMUSE00000170591 Chr2:179912196..179912349 No primer for this exon
downstream ENSMUSE00000170628 Chr2:179911979..179912112 No primer for this exon
downstream ENSMUSE00000170630 Chr2:179911808..179911898 No primer for this exon
downstream ENSMUSE00000170612 Chr2:179911593..179911712 No primer for this exon
downstream ENSMUSE00000378411 Chr2:179911078..179911469 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GGCCAGGTAGGGTTCATTTT Chr2:179946915..179946935 60.19 50 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 TCAACGTCACACTGGACCTG Chr2:179949935..179949955 60.78 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000015647