Tools for the biologist enabling optimized use of gene trap clones

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UniTrap UNI9498
Trapped Gene
Epha2 (ENSMUSG00000006445)
Vector Insertion
Chr 4: 140862497 - 140864319
Public Clones RST288 (baygenomics) RST296 (baygenomics) (ggtc) (ggtc)
IST10774G3 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 5% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000309430 (Chr4:140862429..140862496 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
No primer for this exon TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000309430 (Chr4:140862429..140862496 +)
Downstram Exon
ENSMUSE00000309419 (Chr4:140864320..140864995 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
No primer for this exon No primer for this exon

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000309437 Chr4:140857155..140857349 No primer for this exon
upstream ENSMUSE00000309430 Chr4:140862429..140862496 No primer for this exon

*** Putative Vector Insertion (Chr 4: 140862497 - 140864319) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000309419 Chr4:140864320..140864995 No primer for this exon
downstream ENSMUSE00000309411 Chr4:140872502..140872657 No primer for this exon
downstream ENSMUSE00000309404 Chr4:140872786..140873118 No primer for this exon
downstream ENSMUSE00000309398 Chr4:140874397..140874512 No primer for this exon
downstream ENSMUSE00000309394 Chr4:140874838..140874991 No primer for this exon
downstream ENSMUSE00000309389 Chr4:140876409..140876508 No primer for this exon
downstream ENSMUSE00000309380 Chr4:140877132..140877187 No primer for this exon
downstream ENSMUSE00000309369 Chr4:140877380..140877505 No primer for this exon
downstream ENSMUSE00000381679 Chr4:140877617..140877805 No primer for this exon
downstream ENSMUSE00000350172 Chr4:140877978..140878039 No primer for this exon
downstream ENSMUSE00000309345 Chr4:140878118..140878327 No primer for this exon
downstream ENSMUSE00000524376 Chr4:140878501..140878650 No primer for this exon
downstream ENSMUSE00000183465 Chr4:140879328..140879521 No primer for this exon
downstream ENSMUSE00000309324 Chr4:140880147..140880302 No primer for this exon
downstream ENSMUSE00000360227 Chr4:140884328..140885293 No primer for this exon

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 GAAGGAGGGCTGCACATCTA Chr4:140862530..140862550 60.36 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 ATGGCAAAGGGGTGAGTAAA Chr4:140862487..140862507 59.43 45 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000006445