Tools for the biologist enabling optimized use of gene trap clones

  Homepage | Blast Search | GO Search | Advanced Search | About

UniTrap UNI9634
Trapped Gene
Polr3b (ENSMUSG00000034453)
Vector Insertion
Chr 10: 84162287 - 84176373
Public Clones (sanger) XE414 (baygenomics) W061C01 (ggtc) (ggtc) IST14837H11 (tigm)
Private Clones not available
Severity of mutation (?) Insertion after 80% of polypeptide chain

Suggested RT-PCR (?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000468051 (Chr10:84162144..84162286 +)
Vector Sequence
Forward Primer Location Tm %GC LacZrt Reverse Primer Location
ACAAAGGTGCGACAGACTCC Chr10:84162146..84162165 60.31 55 TGGCGAAAGGGGGATGTG Gene trap vector
 
 
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000468051 (Chr10:84162144..84162286 +)
Downstram Exon
ENSMUSE00000291753 (Chr10:84176374..84176477 +)
Forward Primer Location Tm %GC Reverse Primer Location Tm %GC
ACAAAGGTGCGACAGACTCC Chr10:84162146..84162165 60.31 55 TATGATGTCGGGACAGATGC Chr10:84176447..84176466 59.48 50

Other possible Exon-Primers for RT-PCR (?)
  Exon ID Exon location Forward Primer Primer location Tm %GC
upstream ENSMUSE00000403133 Chr10:84085182..84085283 ATGGATGTTCTAGCCGAGGA Chr10:84085212..84085231 59.65 50
upstream ENSMUSE00000367566 Chr10:84090995..84091027 AGGCTGCTTCCAGCATTTTT Chr10:84091004..84091023 61.25 45
upstream ENSMUSE00000292325 Chr10:84091228..84091284 TAAAGGCCTGGTGAAACAGC Chr10:84091230..84091249 60.25 50
upstream ENSMUSE00000292318 Chr10:84091372..84091436 GCTGACCCCATGTGGTACTT Chr10:84091414..84091433 59.85 55
upstream ENSMUSE00000292314 Chr10:84094530..84094605 TGTCGGACTTCCTGATGTTG Chr10:84094545..84094564 59.68 50
upstream ENSMUSE00000292306 Chr10:84095200..84095300 CGTCTGAGGGACATGACGTA Chr10:84095203..84095222 59.7 55
upstream ENSMUSE00000292301 Chr10:84097646..84097737 CTCCGAAGCTCGAACTGTGT Chr10:84097659..84097678 60.59 55
upstream ENSMUSE00000379048 Chr10:84100800..84100917 CAAGAGCAGCTGTCCAAGAA Chr10:84100844..84100863 59.31 50
upstream ENSMUSE00000426018 Chr10:84102487..84102595 CGTGAAACAGGGTCGATTCTA Chr10:84102523..84102543 60.12 47.62
upstream ENSMUSE00000337154 Chr10:84109541..84109663 AGGAGTGCCAGAAAGCTCAG Chr10:84109626..84109645 59.74 55
upstream ENSMUSE00000385772 Chr10:84115419..84115538 ACCATTCTGACCCACGTACC Chr10:84115518..84115537 59.7 55
upstream ENSMUSE00000291829 Chr10:84118690..84118824 TATGGCAACAAACGACTGGA Chr10:84118792..84118811 60.11 45
upstream ENSMUSE00000291825 Chr10:84130067..84130228 AGATTACCAACGGCATGGTC Chr10:84130194..84130213 59.82 50
upstream ENSMUSE00000291819 Chr10:84136073..84136273 CTCAGTGGGGAATGCTCTGT Chr10:84136230..84136249 60.26 55
upstream ENSMUSE00000291813 Chr10:84137302..84137464 GAACTTGGCCCTCATGACAC Chr10:84137319..84137338 60.52 55
upstream ENSMUSE00000480684 Chr10:84139628..84139781 CCTTACGGATCGCTGTGTTT Chr10:84139731..84139750 60.13 50
upstream ENSMUSE00000484247 Chr10:84142491..84142565 ATGGAAGAGTTGGCTCAAGG Chr10:84142540..84142559 59.28 50
upstream ENSMUSE00000483377 Chr10:84143111..84143209 AACATTGCGCTCTACGAACA Chr10:84143178..84143197 59.49 45
upstream ENSMUSE00000516313 Chr10:84146922..84147049 CCCATACCCTCATCACAACC Chr10:84146982..84147001 60.05 55
upstream ENSMUSE00000514118 Chr10:84155542..84155751 TGGCTACAATCAACGGAACA Chr10:84155549..84155568 60.11 45
upstream ENSMUSE00000465607 Chr10:84158300..84158458 TTTGGGCGTTGTCTTGTGTA Chr10:84158302..84158321 60.15 45
upstream ENSMUSE00000462904 Chr10:84159694..84159811 TAAACAAGTCCATGCCCACA Chr10:84159724..84159743 59.96 45
upstream ENSMUSE00000468051 Chr10:84162144..84162286 ACAAAGGTGCGACAGACTCC Chr10:84162146..84162165 60.31 55

*** Putative Vector Insertion (Chr 10: 84162287 - 84176373) ***

  Exon ID Exon location Reverse Primer Primer location Tm %GC
downstream ENSMUSE00000291753 Chr10:84176374..84176477 TATGATGTCGGGACAGATGC Chr10:84176447..84176466 59.48 50
downstream ENSMUSE00000291749 Chr10:84176990..84177156 GCGAACGAGATCTTCACACA Chr10:84177109..84177128 59.99 50
downstream ENSMUSE00000291740 Chr10:84181155..84181268 GGTAAGGACAGCTCGTGGTC Chr10:84181269..84181288 59.73 60
downstream ENSMUSE00000100019 Chr10:84183089..84183262 CGCTCCATAGCCGATTAGAC Chr10:84183173..84183192 59.83 55
downstream ENSMUSE00000514755 Chr10:84188396..84189922 ACAGGCCCTTGACTGAGCTA Chr10:84189147..84189166 60.01 55

Suggested Genomic-PCR (?)
  Forward Forward location Tm %GC Reverse name Reverse Reverse Location
PCR 1 ACGGCCAGAACACACTAAGG Chr10:84165243..84165263 60.17 55 L232 GATGTGCTGCAAGGCGATTA Gene trap vector
PCR 2 CTAGCGTGACTGGGAAAACC Chr10:84165334..84165354 59.73 55 LacZ CCAGGGTTTTCCCAGTCACG Gene trap vector

Come back to gene ENSMUSG00000034453