Tools for the biologist enabling optimized use of gene trap clones
Homepage
|
Blast Search
|
GO Search
|
Advanced Search
|
About
UniTrap UNI9793
Trapped Gene
Mea1 (
ENSMUSG00000002768
)
Vector Insertion
Chr 17: 46819061 - 46819667
Public Clones
RRC019 (baygenomics)
PST2868-NR (escells)
PST7431-NR (escells)
PST2868-NL (escells)
Private Clones
not available
Severity of mutation
(?)
Insertion after 71% of polypeptide chain
Suggested RT-PCR
(?)
Control PCR for the trapped allele
Upstream Exon
ENSMUSE00000136760 (Chr17:46818958..46819060 +)
Vector Sequence
Forward Primer
Location
Tm
%GC
LacZrt Reverse Primer
Location
No primer for this exon
TGGCGAAAGGGGGATGTG
Gene trap vector
Control PCR for the wild-type allele
Upstream Exon
ENSMUSE00000136760 (Chr17:46818958..46819060 +)
Downstram Exon
ENSMUSE00000424791 (Chr17:46819668..46820049 +)
Forward Primer
Location
Tm
%GC
Reverse Primer
Location
Tm
%GC
No primer for this exon
No primer for this exon
Other possible Exon-Primers for RT-PCR
(?)
Exon ID
Exon location
Forward Primer
Primer location
Tm
%GC
upstream
ENSMUSE00000607108
Chr17:46818073..46818111
No primer for this exon
upstream
ENSMUSE00000136758
Chr17:46818566..46818846
No primer for this exon
upstream
ENSMUSE00000136760
Chr17:46818958..46819060
No primer for this exon
*** Putative Vector Insertion (Chr 17: 46819061 - 46819667) ***
Exon ID
Exon location
Reverse Primer
Primer location
Tm
%GC
downstream
ENSMUSE00000424791
Chr17:46819668..46820049
No primer for this exon
Suggested Genomic-PCR
(?)
Forward
Forward location
Tm
%GC
Reverse name
Reverse
Reverse Location
PCR 1
CCATGGACCCAGGTACAAAA
Chr17:46819050..46819070
60.6
50
L232
GATGTGCTGCAAGGCGATTA
Gene trap vector
PCR 2
CCATGGACCCAGGTACAAAA
Chr17:46819050..46819070
60.6
50
LacZ
CCAGGGTTTTCCCAGTCACG
Gene trap vector
Come back to gene
ENSMUSG00000002768
